The poor digestibility of rapeseed protein is balanced by its very high metabolic utilization in humans
The poor digestibility of rapeseed protein is balanced by its very high metabolic utilization in humans
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DOI：
10.1093/jn/137.3.594
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发表时间：
2007-03-01
影响因子：
4.2
通讯作者：
Gaudichon, Claire
中科院分区：
文献类型：
--
作者：
Bos, Cecile;Airinei, Gheorghe;Gaudichon, Claire
油菜蛋白（RP，Brassica napus）仅用于动物饲料，尽管其对人类营养具有很高的营养潜力。我们试图通过测定饲喂N-15-RP的人的真实的回肠消化率（RID）和餐后蛋白质净利用率（NPPU）来评估油菜籽的营养品质。在空肠（n = 5）或回肠（n = 7）水平配备肠管的志愿者摄入含有27.3 g N-15-RP和700 kcal（2.93 MJ）总能量含量的混合膳食。在餐后定期取样的肠液、尿液和血液中定量测定饮食氮动力学。RP的RID为84.0 ± 8.8%。日粮氮素主要以12 S和2S菜籽组分中未消化蛋白质的形式存在。膳食摄入不会显著增加氨基酸血症。膳食氮的餐后分布为5.4 +/- 1.8%的尿尿素和氨，8.2 +/- 3.4%的体内尿素，和7.7 +/- 2.0%的血浆蛋白餐后8小时。RP的NPPU为70.5 +/- 9.6%，餐后生物学值（PBV）高达83.8 +/-4.6%。与其他植物蛋白相比，RP在人体中具有低RID，但也表现出非常低的脱氨基速率。因此，RP的PBV在人体中是极好的，与乳蛋白的PBV一样高。我们得出结论，RP具有很高的营养潜力，为人类营养。
Rapeseed protein (RP, Brassica napus) is used in only animal feed despite its high nutritional potential for human nutrition. We sought to assess the nutritional quality of rapeseed by measuring its real ileal digestibility (RID) and net postprandial protein utilization (NPPU) in humans fed N-15-RP. Volunteers equipped with an intestinal tube at the jejunal (n = 5) or ileal level (n = 7) ingested a mixed meal containing 27.3 g N-15-RP and a total energy content of 700 kcal (2.93 MJ). Dietary N kinetics was quantified in intestinal fluid, urine, and blood sampled at regular intervals during the postprandial period. The RID of RP was 84.0 +/- 8.8%. Dietary N at the deal level was mostly in the form of undigested protein from both 12S and 2S rapeseed fractions. Aminoacidemia was not significantly increased by meal ingestion. The postprandial distribution of dietary N was 5.4 +/- 1.8% in urinary urea and ammonia, 8.2 +/- 3.4% in body urea, and 7.7 +/- 2.0% in plasma protein 8 h after the meal. The NPPU of RP amounted to 70.5 +/- 9.6% and the postprandial biological value (PBV) was high at 83.8 +/- 4.6%. RP has a low RID in humans compared with other plant proteins but also exhibits a very low deamination rate. Thus, the PBV of RP is excellent in humans, being as high as that of milk protein. We conclude that RP has a high nutritional potential for human nutrition.