Time-related bisphenol-A content and estrogenic activity in saliva samples collected in relation to placement of fissure sealants.
Time-related bisphenol-A content and estrogenic activity in saliva samples collected in relation to placement of fissure sealants.
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DOI：
10.1007/s007840050089
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发表时间：
1999-09-01
影响因子：
3.4
通讯作者：
Schmalz, G
中科院分区：
文献类型：
--
作者：
Arenholt-Bindslev, D;Breinholt, V;Schmalz, G
最近有报道称，在放置一种裂缝密封剂（Delton）后1小时内收集的唾液样本中检测到雌激素活性，这与双酚a （BPA）含量有关。本研究的目的是确定在放置两种不同单体成分的裂缝密封剂前后收集的唾液样本中与时间相关的BPA含量和雌激素活性。8名健康男性志愿者没有使用过牙缝填充物或复合树脂填充物的历史，他们用Delton LC（4人）或Visio-Seal（4人）封闭了4颗磨牙。实验前，在早晨禁食时收集基线唾液样本。放置裂缝密封剂，并在放置裂缝密封剂1 h和24 h后立即收集唾液样本。在Delton LC放置后立即收集的唾液样本中发现了BPA（范围0.3-2.8 ppm）。Delton处理后1 h和24 h未检测到BPA的含量（检测限<或= 0.1 ppm）。在基线样本和从Visio-Seal处理个体收集的所有样本中，未检测到双酚a。在重组酵母细胞实验中，在Delton LC密封胶放置后立即收集的唾液样本中发现雌激素活性显著增加（P < 0.05；方差分析），而在其余组中没有观察到统计学上显著的雌激素活性。总之，在Delton LC裂缝密封剂放置后1小时和24小时收集的唾液样本中检测到微量的BPA，但比先前报道的要低得多。使用Visio-Seal缝隙密封胶后未检测到BPA。
It was recently reported that estrogenic activity was detected in saliva samples collected during 1 h after placement of one fissure sealant (Delton) and this related to Bisphenol-A (BPA) content. The aim of the present study was to determine the time-related BPA content and estrogenic activity in saliva samples collected before and after placement of two fissure sealants each with a different monomer composition. Eight healthy male volunteers with no history of prior placement of fissure sealants or composite resin fillings had four molars sealed with either Delton LC (four people) or Visio-Seal (four people). Base-line saliva samples were collected preexperimentally, in the morning when fasting. Fissure sealants were placed and saliva samples collected immediately, 1 h and 24 hs after placement of the fissure sealant. BPA was found in saliva samples collected immediately after placement of Delton LC (range 0.3-2.8 ppm). No detectable amounts of BPA were determined 1 h and 24 h after Delton treatment (detection limit < or = 0.1 ppm). In base-line samples and in all samples collected from Visio-Seal treated individuals, no BPA was detected. In a recombinant yeast cell assay, significantly increased estrogenic activity was found in saliva samples collected immediately after placement of Delton LC sealant (P < 0.05; ANOVA) whereas no statistically significant estrogenic activity was observed in the remaining groups. In conclusion, minute amounts of BPA, however considerably lower than previously reported, were detected in saliva samples collected immediately after but not 1 and 24 h(s) after placement of Delton LC fissure sealant. BPA was not detected after placement of Visio-Seal fissure sealant.