Naturally acquired inhibitory antibodies to Plasmodium vivax Duffy binding protein are short-lived and allele-specific following a single malaria infection
Naturally acquired inhibitory antibodies to Plasmodium vivax Duffy binding protein are short-lived and allele-specific following a single malaria infection
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DOI：
10.1111/j.1365-2249.2009.03931.x
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发表时间：
2009-06-01
影响因子：
4.6
通讯作者：
Carvalho, L. H.
中科院分区：
文献类型：
--
作者：
Ceravolo, I. P.;Sanchez, B. A. M.;Carvalho, L. H.
间日疟原虫（Plasmodium vivax， DBP）的Duffy结合蛋白（Duffy binding protein）是一种重要的黏附配体，参与人Duffy阳性红细胞的分裂子侵袭。在巴西非疟疾地区的一个村庄，间日疟原虫疟疾的小规模暴发为我们提供了一个机会，调查首次和短暂接触疟疾的个体的DBP免疫反应。33人参加了5次横断面调查，其中15人在疫情暴发地区居住时确诊间日疟感染（病例），18人没有经历过疟疾（非病例）。在本研究中，我们发现首次感染间日疟原虫的个体中只有20%（15人中有3人）对DBP产生抗体反应；复发间日疟原虫感染可实现二次增强。来自初发/复发间日疟原虫样本的DNA序列在暴发地区的样本中发现了单个dbp等位基因。为了研究DBP配体结构域（富半胱氨酸区II， DBPII）的抑制性抗体，我们用表达DBPII序列的哺乳动物细胞进行了体外实验，这些序列与爆发分离的DBPII序列同源或不同源。在非免疫个体中，12个月随访期的结果提供了证据，证明自然获得的DBPII抑制抗体是短暂的，并且偏向于特定的等位基因。
The Duffy binding protein of Plasmodium vivax (DBP) is a critical adhesion ligand that participates in merozoite invasion of human Duffy-positive erythrocytes. A small outbreak of P. vivax malaria, in a village located in a non-malarious area of Brazil, offered us an opportunity to investigate the DBP immune responses among individuals who had their first and brief exposure to malaria. Thirty-three individuals participated in the five cross-sectional surveys, 15 with confirmed P. vivax infection while residing in the outbreak area (cases) and 18 who had not experienced malaria (non-cases). In the present study, we found that only 20% (three of 15) of the individuals who experienced their first P. vivax infection developed an antibody response to DBP; a secondary boosting can be achieved with a recurrent P. vivax infection. DNA sequences from primary/recurrent P. vivax samples identified a single dbp allele among the samples from the outbreak area. To investigate inhibitory antibodies to the ligand domain of the DBP (cysteine-rich region II, DBPII), we performed in vitro assays with mammalian cells expressing DBPII sequences which were homologous or not to those from the outbreak isolate. In non-immune individuals, the results of a 12-month follow-up period provided evidence that naturally acquired inhibitory antibodies to DBPII are short-lived and biased towards a specific allele.