Glial imaging during synapse remodeling at the neuromuscular junction.
Glial imaging during synapse remodeling at the neuromuscular junction.
复制标题
神经肌肉接头处突触重塑过程中的神经胶质成像。
DOI：
10.1017/s1740925x09990421
复制
发表时间：
2008
影响因子：
--
通讯作者：
Bishop,Derron
中科院分区：
文献类型：
--
作者：
Zuo,Yi;Bishop,Derron
胶质细胞是突触不可或缺的结构和功能成分。它们调节突触传递，也在突触的形成和维持中发挥重要作用。脊椎动物神经肌肉接头(NMJ)是一种经典的突触模型。由于其体积大、简单和可获得性，NMJ对我们理解突触的发育和组织做出了巨大贡献。在过去的十年中，NMJ也成为研究神经胶质细胞-突触相互作用的有效模型，部分原因是各种标记技术的发展，使得NMJ及其相关的雪旺细胞(NMJ处的胶质细胞)可以在体外和体内可视化。这些研究表明，雪旺细胞在突触发育早期和损伤后的神经再支配过程中都参与了突触的重构。活体成像也与连续切片的透射电子显微镜重建相结合，以直接观察改建的NMJ的超微结构。在本综述中，我们利用目前可用的最高时间和空间分辨率方法，着重于雪旺细胞动力学及其在脊椎动物NMJ的形成、成熟和重塑中的作用的解剖学研究。
Glia are an indispensable structural and functional component of the synapse. They modulate synaptic transmission and also play important roles in synapse formation and maintenance. The vertebrate neuromuscular junction (NMJ) is a classic model synapse. Due to its large size, simplicity and accessibility, the NMJ has contributed greatly to our understanding of synapse development and organization. In the past decade, the NMJ has also emerged as an effective model for studying glia–synapse interactions, in part due to the development of various labeling techniques that permit NMJs and associated Schwann cells (the glia at NMJs) to be visualized in vitro and in vivo. These approaches have demonstrated that Schwann cells are actively involved in synapse remodeling both during early development and in post-injury reinnervation. In vivo imaging has also recently been combined with serial section transmission electron microscopic (ssTEM) reconstruction to directly examine the ultrastructural organization of remodeling NMJs. In this review, we focus on the anatomical studies of Schwann cell dynamics and their roles in formation, maturation and remodeling of vertebrate NMJs using the highest temporal and spatial resolution methods currently available.