MONOCLONAL-ANTIBODIES TO MURINE HEPATITIS VIRUS-4 (STRAIN-JHM) DEFINE THE VIRAL GLYCOPROTEIN RESPONSIBLE FOR ATTACHMENT AND CELL CELL-FUSION
MONOCLONAL-ANTIBODIES TO MURINE HEPATITIS VIRUS-4 (STRAIN-JHM) DEFINE THE VIRAL GLYCOPROTEIN RESPONSIBLE FOR ATTACHMENT AND CELL CELL-FUSION
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DOI：
10.1016/0042-6822(82)90095-2
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发表时间：
1982-01-01
期刊：
影响因子：
3.7
通讯作者：
BUCHMEIER, MJ
中科院分区：
文献类型：
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作者：
COLLINS, AR;KNOBLER, RL;BUCHMEIER, MJ
建立了产生小鼠肝炎病毒-4 (MHV-4) JHM株单克隆抗体的小鼠杂交瘤细胞株。采用间接免疫荧光法和免疫沉淀法鉴定了3种病毒多肽特异性单克隆抗体。核衣壳单克隆抗体在感染小鼠L-241细胞的细胞质中反应，沉淀出60000道尔顿（60K）的MHV-4核衣壳多肽（VP-4）。其他单克隆抗体在细胞质和感染细胞表面反应并沉淀170K病毒糖蛋白（GP-1）。第三组单克隆抗体在细胞质和感染细胞表面发生反应，沉淀25K病毒糖蛋白（GP-5）及其前体VP-6 （23K）。抗gp -1单独对MHV-4病毒具有直接中和活性，而在补体存在时，抗gp -1和抗gp -5均能中和病毒。在L241细胞中，只有抗gp -1具有抑制融合感染扩散的能力。因此，病毒糖蛋白GP-1可能同时具有MHV-4的附着和融合活性。
Mouse hybridoma cell lines producing monoclonal antibodies to the JHM strain of mouse hepatitis virus-4 (MHV-4) were established. By indirect immunofluorescence and immune precipitation, monoclonal antibodies of 3 viral polypeptide specificities were characterized. Monoclonal antibodies to nucleocapsid reacted in the cytoplasm of infected mouse L-241 cells and precipitated the 60,000 dalton (60K) nucleocapsid polypeptide (VP-4) of MHV-4. Other monoclonal antibodies reacted both in the cytoplasm and on the surface of infected cells and precipitated the 170K viral glycoprotein (GP-1). A 3rd set of monoclonal antibodies reacted both in the cytoplasm and on the surface of infected cells and precipitated the 25K viral glycoprotein (GP-5) and its precursor VP-6 (23K). Anti-GP-1 alone had direct neutralizing activity for MHV-4 virus, while in the presence of complement both anti-GP-1 and anti-GP-5 neutralized virus. Only anti-GP-1 had the ability to inhibit the spread of infection due to fusion in L241 cells. Thus, the viral glycoprotein GP-1 likely contains both the attachment and fusion activities of MHV-4.