A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs
A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs
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DOI：
10.3390/mi11030246
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发表时间：
2020-03-01
期刊：
影响因子：
3.4
通讯作者：
Thiele, Julian
中科院分区：
文献类型：
--
作者：
Maennel, Max J.;Fischer, Carolin;Thiele, Julian
微流体装置的三维（3D）打印不断取代传统的制造方法。实现微观特征尺寸和短工艺时间的通用工具是微立体光刻（mu SL）。然而，用于mu SL的普通树脂缺乏生物相容性并且具有细胞毒性。这项工作的重点是开发新的光固化树脂的基础上，为μ SL制造的聚合物材料和表面的细胞培养。筛选不同的基于丙烯酸酯和甲基丙烯酸酯的组合物的材料特性，包括润湿性、表面粗糙度和溶胀行为。为了进一步了解，研究了光吸收剂和光引发剂对3D打印基底细胞毒性的影响。将标准聚苯乙烯容器中的人脐静脉内皮细胞（HUVEC）的细胞培养实验与我们自制树脂库制成的3D打印部件进行比较。其中，在优化材料组成和后处理后，我们确定了聚（乙二醇）二丙烯酸酯（PEGDA）和聚（乙二醇）甲基丙烯酸甲酯（PEGMEMA）的混合物最适合用于制造细胞培养平台，保留HUVEC的活力和增殖。接下来，我们的PEGDA/PEGMEMA树脂将在最小特征尺寸和细胞粘附方面进一步优化，以制造微观（微流体）细胞培养平台，例如，用于体外研究HUVECs的血管形成。
Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (mu SL). However, common resins for mu SL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for mu SL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.