WASP: allele-specific software for robust molecular quantitative trait locus discovery.
WASP: allele-specific software for robust molecular quantitative trait locus discovery.
复制标题
DOI：
10.1038/nmeth.3582
复制
发表时间：
2015-11
期刊：
影响因子：
48
通讯作者：
Pritchard JK
中科院分区：
文献类型：
--
作者：
van de Geijn B;McVicker G;Gilad Y;Pritchard JK
等位基因特异性测序读数为鉴定分子数量性状基因座（QTL）提供了强有力的信号，然而它们具有分析的挑战性并且易于出现技术假象。在这里，我们描述了WASP，一套工具，用于无偏等位基因特异性读取作图和分子QTL的发现。使用模拟读数，RNA-seq读数和ChIP-seq读数，我们证明了WASP具有低错误率，并且比现有的QTL定位方法更强大。
Allele-specific sequencing reads provide a powerful signal for identifying molecular quantitative trait loci (QTLs), however they are challenging to analyze and prone to technical artefacts. Here we describe WASP, a suite of tools for unbiased allele-specific read mapping and discovery of molecular QTLs. Using simulated reads, RNA-seq reads and ChIP-seq reads, we demonstrate that WASP has a low error rate and is far more powerful than existing QTL mapping approaches.