Estimation of movement from insectary plants to crop plants in Orius bugs (Heteroptera: Anthocoridae) by molecular gut content analysis
Estimation of movement from insectary plants to crop plants in Orius bugs (Heteroptera: Anthocoridae) by molecular gut content analysis
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DOI：
10.1007/s13355-020-00692-9
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发表时间：
2020-06-26
影响因子：
1.3
通讯作者：
Miura, Kazuki
中科院分区：
文献类型：
--
作者：
Hayashi, Masayuki;Abe, Junichiro;Miura, Kazuki
在农业生态系统中，广泛采用昆虫和银行家植物的引入来提高害虫节肢动物天敌的生存和繁殖。被昆虫植物吸引和/或维持在昆虫植物上的天敌需要从昆虫植物移动到作物植物以有效地抑制害虫种群。然而，在许多情况下，缺乏支持天敌迁移的直接证据。为了研究在生物防治中具有重要作用的植食性天敌--小花蝽的运动规律，我们利用DNA分子标记技术对小花蝽的肠道内容物进行了分析。设计特异性PCR引物，对昆虫植物海草菇DNA进行检测。我们还利用被捕食昆虫的DNA标记进行了肠道内容物分析，以估计Oriusbugs的捕食谱。在茄子叶片上采集的小花蝽腹部检测到昆虫植物的DNA，表明小花蝽消耗了昆虫植物的食物资源，并迁移到了作物植物上。在茄子田的小花蝽中检测到3种被捕食昆虫的DNA，即棕榈蓟马（Thrips palmiandFrankliniella intonsa）和棉蚜（Aphis gossypii），表明小花蝽对它们有捕食作用。
The introduction of insectary and banker plants is widely used in agroecosystems to improve the survival and reproduction of natural enemies of pest arthropods. Natural enemies that are attracted to and/or maintained on insectary plants need to move from the insectary plants to crop plants to suppress pest populations effectively. In many cases, however, direct evidence supporting the migration of natural enemies is lacking. To investigate the movement ofOriusbugs (Hemiptera: Anthocoridae), which are efficient zoophytophagous predators used in biological control, we performed gut content analyses based on DNA markers. Specific PCR primers were designed to detect the DNA of the insectary plant,Scaevola aemula. We also carried out gut content analyses using prey insect DNA markers to estimateOriusbugs' predation spectrum. DNA of the insectary plant was detected in the abdomen ofOriusbugs collected on eggplant leaves, suggesting thatOriusbugs had consumed food resources from the insectary plants and relocated to the crop plants. DNA of three prey insect species-two thrips species,Thrips palmiandFrankliniella intonsa, and cotton aphid,Aphis gossypii-was detected inOriusbugs in the eggplant field, indicating thatOriusbugs preyed on them.