Relationship between the flexibility and the motility of actin filaments: Effects of pH
Relationship between the flexibility and the motility of actin filaments: Effects of pH
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DOI：
10.1016/j.bbrc.2008.04.135
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发表时间：
2008-07-11
影响因子：
3.1
通讯作者：
Hatori, Kuniyuki
中科院分区：
文献类型：
--
作者：
Arii, Yusuke;Hatori, Kuniyuki
以荧光标记的肌动蛋白细丝的滑动速度和持续长度作为衡量肌动蛋白细丝弯曲柔韧性的指标，通过改变溶液的pH值进行了运动性测定。在组成单体形成肌动蛋白细丝的溶液中，当pH值从5.0变化到9.0时，与钙离子结合的肌动蛋白微丝(Ca-F-肌动蛋白)相比，与镁离子结合的肌动蛋白微丝(MG-F-肌动蛋白)相比，镁离子结合的肌动蛋白微丝的运动性能明显受到抑制。Ca-F-肌动蛋白的持续长度随pH值的增加而逐渐增加，而镁-F-肌动蛋白的类似长度与pH值无关。而镁-氟-肌动蛋白和钙-氟-肌动蛋白的最大滑移速度都是在约6微米的持续长度处获得的。(C)2008 Elsevier Inc.保留所有权利。
Both the sliding velocity of fluorescently labeled actin filament and its persistence length as an index of the bending flexibility of the filament were examined in the motility assay as varying the pH values of the solution for preparing actin filaments. When the pH value was varied from 5.0 to 9.0 in the solution in which actin filaments were formed from the constituent monomers, the motile performance of Mg2+ bound actin filaments (Mg-F-actin) was apparently suppressed compared to the case of Ca2+ bound ones (Ca-F-actin). The persistence length for Ca-F-actin gradually increased with the increase of the pH value while the similar length for Mg-F-actin remained rather independent of the value. The largest sliding velocity of the filament, on the other hand, obtained at the persistence length of roughly 6 mu m for both cases of Mg-F-actin and Ca-F-actin. (c) 2008 Elsevier Inc. All rights reserved.