Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster.
Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster.
复制标题
果蝇玫瑰色位点异色位置效应机制研究
DOI：
10.1093/genetics/108.3.603
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发表时间：
1984
期刊：
影响因子：
3.3
通讯作者：
Chovnick,A
中科院分区：
文献类型：
--
作者：
Rushlow,CA;Bender,W;Chovnick,A
描述了扩展玫瑰色轨迹的位置效应变体的表征并测试异色位置效应的可能机制的实验。玫瑰色位置效应变体在马氏管中黄嘌呤脱氢酶活性方面表现出多样化的表型。位置效应突变的断点位于玫瑰色基因座DNA外的玫瑰色区域的DNA图谱上，ryps1136位于紧邻玫瑰色的thel（3）S12基因的DNA中，而ryps1149位于piclocus中玫瑰色的远侧约15kb处。Southern印迹实验测试和拒绝的概念，异染色质位置效应的结果，从复制不足的位置受影响的基因。相反，北方印迹的结果有助于将注意力引向作为转录缺陷的位置效应。组蛋白区域缺失杂合性和丁酸喂养实验未能表现出特定的抑制玫瑰色位点的位置效应。
Experiments are described that extend the characterization of position effect variants of the rosy locus and test possible mechanisms of heterochromatic position effect.—Rosy position effect variants exhibit a variegated phenotype with respect to xanthine dehydrogenase activity in malpighian tubules.—The breakpoints of the position effect mutations are located on the DNA map of the rosy region outside of the rosy locus DNA;ryps1136is located in the DNA of thel(3)S12gene immediately proximal to rosy, whereasryps1149is located some 15 kb distal to rosy in thepiclocus.—Southern blot experiments are described that test and reject the notion that heterochromatic position effect results from underreplication of the position-affected gene. Rather, the results of Northern blots serve to direct attention to position effect as a defect in transcription.—Histone region deletion heterozygosity and butyrate-feeding experiments failed to exhibit specific suppression of position effect at the rosy locus.