Nutraceutical intervention reverses the negative effects of blood from aged rats on stem cells
Nutraceutical intervention reverses the negative effects of blood from aged rats on stem cells
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DOI：
10.1007/s11357-015-9840-7
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发表时间：
2015-10-01
期刊：
影响因子：
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通讯作者：
Shytle, R. Douglas
中科院分区：
文献类型：
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作者：
Bickford, Paula C.;Kaneko, Yuji;Shytle, R. Douglas
衰老与身体许多干细胞功能的下降有关。大量文献表明，这种功能下降的原因之一是细胞对身体生态位的非自主影响。例如，使用联体共生技术的研究表明，老年小鼠的血液对年轻小鼠的肌肉卫星细胞和神经发生有负面影响。我们研究了是否可以通过用 NT-020 治疗老年大鼠来逆转老年血清对干细胞增殖的影响，NT-020 是一种含有蓝莓、绿茶、维生素 D3 和肌肽的膳食补充剂，已被证明可以增加老年大鼠的神经发生。给年轻和年老的大鼠喂食对照 NIH-31 饮食或补充 NT-020 饮食 28 天，并在安乐死后收集血清。该血清用于培养大鼠海马神经祖细胞（NPC）和大鼠骨髓源性间充质干细胞（MSC）。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑 (MTT) 和 5-溴-2'-脱氧尿苷 (BrdU) 检测，在 NPC 和 MSC 中检测到老年大鼠的血清显着降低了细胞增殖。用 NT-020 处理的老年大鼠的血清与年轻大鼠的血清没有不同。因此，NT-020挽救了老年大鼠血清减少干细胞增殖的作用。
Aging is associated with a decline in function in many of the stem cell niches of the body. An emerging body of literature suggests that one of the reasons for this decline in function is due to cell non-autonomous influences on the niche from the body. For example, studies using the technique of parabiosis have demonstrated a negative influence of blood from aged mice on muscle satellite cells and neurogenesis in young mice. We examined if we could reverse this effect of aged serum on stem cell proliferation by treating aged rats with NT-020, a dietary supplement containing blueberry, green tea, vitamin D3, and carnosine that has been shown to increase neurogenesis in aged rats. Young and aged rats were administered either control NIH-31 diet or one supplemented with NT-020 for 28 days, and serum was collected upon euthanasia. The serum was used in cultures of both rat hippocampal neural progenitor cells (NPCs) and rat bone marrow-derived mesenchymal stem cells (MSCs). Serum from aged rats significantly reduced cell proliferation as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-bromo-2'-deoxyuridine (BrdU) assays in both NPCs and MSCs. Serum from aged rats treated with NT-020 was not different from serum from young rats. Therefore, NT-020 rescued the effect of serum from aged rats to reduce stem cell proliferation.