Aggregate Size and Architecture Determine Microbial Activity Balance for One-Stage Partial Nitritation and Anammox
Aggregate Size and Architecture Determine Microbial Activity Balance for One-Stage Partial Nitritation and Anammox
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DOI：
10.1128/aem.02337-09
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Verstraete, Willy
中科院分区：
文献类型：
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作者：
Vlaeminck, Siegfried E.;Terada, Akihiko;Verstraete, Willy
好氧氨氧化细菌(AerAOB)和缺氧氨氧化细菌(AnAOB)在部分亚硝化/厌氧氨氧化系统中协同作用去除废水中的氨氮。在这个过程中，大颗粒微生物聚集体提高了性能，但到目前为止对颗粒化的了解很少。本研究采用了3个悬浮生长的限氧自养硝化反硝化(OLAND)反应器，分别命名为A、B、C三个不同接种和运行条件(混合和曝气)的反应器。试验目标是(I)量化不同集料尺寸下AerAOB和AnAOB的丰度和活性平衡；(Ii)推定集料形态、尺寸分布和结构与三个反应器的接种和运行之间的关系。亚硝酸盐累积速率比(NIR)定义为净好氧亚硝酸盐产生率除以缺氧性亚硝酸盐消耗率。反应器A、B和C的最小聚集体是亚硝酸盐来源(NARR，>；1.7)。反应器A和C的大型集合体是能够自主脱氮的颗粒颗粒(NORR为0.6~1.1)，内部ANAOB区被AerAOB边缘包围。这些颗粒中约50%的自养空间由AerAOB和AnAOB特异的胞外聚合物组成。大的反应器B聚集体是薄膜状的亚硝酸盐汇(NARR，<；0.5)，其中的AnAOB没有被AerAOB层屏蔽。孔洞和通道占据了富含AnAOB的聚集体(B和C反应器)缺氧区的13%到17%。假设的颗粒化途径包括通过分裂和发芽进行颗粒复制，并由基于物种特定生理的生长和/或衰退以及流体动力剪切和混合驱动。
Aerobic ammonium-oxidizing bacteria (AerAOB) and anoxic ammonium-oxidizing bacteria (AnAOB) cooperate in partial nitritation/anammox systems to remove ammonium from wastewater. In this process, large granular microbial aggregates enhance the performance, but little is known about granulation so far. In this study, three suspended-growth oxygen-limited autotrophic nitrification-denitrification (OLAND) reactors with different inoculation and operation (mixing and aeration) conditions, designated reactors A, B, and C, were used. The test objectives were (i) to quantify the AerAOB and AnAOB abundance and the activity balance for the different aggregate sizes and (ii) to relate aggregate morphology, size distribution, and architecture putatively to the inoculation and operation of the three reactors. A nitrite accumulation rate ratio (NARR) was defined as the net aerobic nitrite production rate divided by the anoxic nitrite consumption rate. The smallest reactor A, B, and C aggregates were nitrite sources (NARR, > 1.7). Large reactor A and C aggregates were granules capable of autonomous nitrogen removal (NARR, 0.6 to 1.1) with internal AnAOB zones surrounded by an AerAOB rim. Around 50% of the autotrophic space in these granules consisted of AerAOB- and AnAOB-specific extracellular polymeric substances. Large reactor B aggregates were thin film-like nitrite sinks (NARR, < 0.5) in which AnAOB were not shielded by an AerAOB layer. Voids and channels occupied 13 to 17% of the anoxic zone of AnAOB-rich aggregates (reactors B and C). The hypothesized granulation pathways include granule replication by division and budding and are driven by growth and/or decay based on species-specific physiology and by hydrodynamic shear and mixing.