Biospeckle Optical Coherence Tomography (bOCT) reveals variable effects of acid mine drainage (AMD) on monocot and dicot seed germination
Biospeckle Optical Coherence Tomography (bOCT) reveals variable effects of acid mine drainage (AMD) on monocot and dicot seed germination
复制标题
生物斑点光学相干断层扫描 (bOCT) 揭示了酸性矿山排水 (AMD) 对单子叶植物和双子叶植物种子萌发的不同影响
DOI：
10.1117/12.2621440
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
De Silva Yakdehige Sanath Kumara
中科院分区：
文献类型：
--
作者：
Li Danyang;Rajagopalan Uma Maheswari;Kadono Hirofumi;De Silva Yakdehige Sanath Kumara
酸性矿山废水(AMD)是在矿物开采过程中产生的。AMD污染农田和河流，因此研究AMD对农作物的影响是有意义的。针对传统的植物对环境胁迫反应的研究方法破坏性大、耗时长的问题，我们提出了一种快速、非侵入性的植物AMD反应的生物斑点OCT(BOCT)方法。在本研究中，我们选择水稻植株和大豆作为实验对象。将种子分别置于40和80ml/L的模拟AMD溶液中，用BOCT对种子进行监测。这些种子的OCT图像以每秒10帧的速度获得，持续了几十秒。对于OCT结构图像的每个像素，计算时间轴上的对比度以得到BOCT图像。同时，我们测量了水稻和大豆在AMD上生长后的根冠长度作为对比。结果表明，在较短的时间内，大豆和水稻经40和80ml/L的AMD处理后的种子生物活性变化与对照相比，BOCT图像明显区分开来，而常规方法在相同时间内没有显示任何变化。大豆和水稻的幼苗生长状况分别在7d和10d后表现出与BOCT结果相同的趋势。这表明所提出的BOCT方法可以在很早的阶段揭示大豆和水稻对不同浓度AMD的不同反应。该技术可为研究植物对环境胁迫的响应提供参考指标，且具有高效、非侵入性等特点。
Acid mine drainage (AMD) is generated during the mineral extraction process. AMD contaminates farmland and rivers, so it makes sense to study the effects of AMD on crops. Since the conventional methods for studying plant responses to environmental stress are damaging and time-consuming, we propose the Biospeckle OCT (bOCT) method to evaluate the response of plants to AMD in a rapid and non-invasive way. In this study, we selected rice plant and soybean as experimental subjects. The seeds were exposed to 40 and 80 ml/L of simulated AMD solution, and the seed condition was monitored by bOCT. OCT images of the seeds were obtained at a speed of 10 frames per second for a few tens of seconds. For each pixel of the OCT structural images, the contrast across the temporal axis was calculated to give bOCT images. Meanwhile, we measured the root shoot length of rice and soybean after growing in AMD as a comparison. It was found that bOCT images clearly distinguished the changes in biological activities of seeds due to 40 and 80 ml/L of AMD treatments from those of control within much shorter time, 48 hours and 72 hours for soybean and rice, respectively, compared to the conventional method that failed to show any changes within the same time. And The seedling growing status of soybean and rice after 7 and 10 days showed the same trend as the bOCT results, respectively. This suggests that the proposed bOCT method can reveal the different responses of soybean and rice to different concentrations of AMD at a very early stage. This technique may be able to provide a reference indicator for studying plant response to environmental stress, and it is efficient and non-invasive.