Subcutaneous inoculation of a whole virus particle vaccine prepared from a non-pathogenic virus library induces protective immunity against H7N7 highly pathogenic avian influenza virus in cynomolgus macaques
Subcutaneous inoculation of a whole virus particle vaccine prepared from a non-pathogenic virus library induces protective immunity against H7N7 highly pathogenic avian influenza virus in cynomolgus macaques
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DOI：
10.1016/j.vaccine.2009.10.067
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发表时间：
2010-01-08
期刊：
影响因子：
5.5
通讯作者：
Ogasawara, Kazumasa
中科院分区：
文献类型：
--
作者：
Itoh, Yasushi;Ozaki, Hiroichi;Ogasawara, Kazumasa
H7N7高致病性禽流感病毒（HPAIV）疫苗的开发是一个紧迫的问题，因为已有人感染该亚型病毒的病例报道，而且大多数人对H7N7病毒没有免疫力。我们结合非致病性甲型流感病毒的流感病毒库中的成分制成了 H7N7 疫苗。通过在食蟹猴中皮下接种全病毒颗粒疫苗（有或没有明矾作为佐剂），可引发针对疫苗株的特异性抗体和 T 细胞回忆反应。在接种明矾和不接种明矾之间，抗体反应的强度没有观察到显着差异，尽管接种明矾的疫苗比不接种明矾的疫苗接种诱导了更长的CD8(+)T细胞回忆反应。在用 H7N7 HPAIV 亚型攻击后，在未接种疫苗的猕猴鼻拭子中检测到病毒长达 8 天，但在接种或不接种明矾的动物中仅检测到 I 天，尽管接种明矾的猕猴在感染后更短暂地表现出体温升高。这些发现表明，这种 H7N7 HPAIV 毒株对猕猴具有致病性，并且该疫苗赋予猕猴针对 H7N7 HPAIV 感染的保护性免疫力。 (C) 2009 Elsevier Ltd. 保留所有权利。
Development of H7N7 highly pathogenic avian influenza virus (HPAIV) vaccines is an urgent issue since human cases of infection with this subtype virus have been reported and most humans have no immunity against H7N7 viruses. We made an H7N7 vaccine combining components from an influenza virus library of non-pathogenic type A influenza viruses Antibody and T cell recall responses specific against the vaccine strain were elicited by subcutaneous inoculation with the whole virus particle vaccine with or without alum as an adjuvant in cynomolgus macaques. No significant difference was observed in magnitude of antibody responses between vaccination with alum and vaccination without alum, though vaccination with alum induced longer recall responses of CD8(+) T cells than did vaccination without alum. After challenge with a subtype of H7N7 HPAIV, the virus was detected in nasal swabs of unvaccinated macaques for 8 days but only for I day in the animals vaccinated either with or without alum, although the macaques vaccinated with alum showed elevated body temperature more briefly after infection. These findings demonstrated that this H7N7 HPAIV strain is pathogenic to macaques and that the vaccine conferred protective immunity to macaques against H7N7 HPAIV infection. (C) 2009 Elsevier Ltd. All rights reserved.