Expression levels of NPPB, ITGB6, CPNE4, EML5, and ITSN1 in fresh exudates swabbed from critically colonised and infected full-thickness wounds in rats.
Expression levels of NPPB, ITGB6, CPNE4, EML5, and ITSN1 in fresh exudates swabbed from critically colonised and infected full-thickness wounds in rats.
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NPPB，ITGB6，CPNE4，EML5和ITSN1的表达水平在大鼠的批判性殖民和感染的全厚性伤口中擦拭。
DOI：
10.1111/iwj.13965
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发表时间：
2023-04
影响因子：
3.1
通讯作者：
Sanada, Hiromi
中科院分区：
文献类型：
--
作者：
Takizawa, Chihiro;Minematsu, Takeo;Nakagami, Gojiro;Kitamura, Aya;Koudounas, Sofoklis;Kunimitsu, Mao;Sanada, Hiromi
由于缺乏感染迹象，压力损伤管理需要可靠地识别关键的定植。我们的研究组曾建议将mRNAs利钠肽B(NPPB)、整合素亚单位β6(Itgb6)、Copine 4(Cpne4)、棘皮微管相关蛋白5和交叉素1作为严重定植创面渗出液的候选标记物。然而，目前尚不清楚候选基因的mRNAs或蛋白质是否适合作为新鲜渗出物的生物标志物。因此，本研究旨在评估mRNAs和蛋白质作为新的渗出物标志物用于关键定植的有效性。在大鼠身上建立了正常愈合、严重定植和感染三种伤口模型。收集新鲜的拭子分泌物，并测定其mRNA和蛋白的表达水平。在新鲜创面渗出液中，Itgb6在危重和感染创面中的检出频率呈下降趋势(P=0.067)，而Cpne4和NPPB在感染创面中的检出频率往往低于正常愈合和危重定植创面(P值分别为0.006和0.067)。相反，渗出液中的蛋白表达没有差异。这项研究表明，新鲜渗出物中的Itgb6mRNA是一种有希望的关键定植的生物标志物。
Pressure injury management requires reliable identification of critical colonisation due to lack of infection signs. Our research group previously proposed the mRNAs natriuretic peptide B (Nppb), integrin subunit beta 6 (Itgb6), copine 4 (Cpne4), echinoderm microtubule‐associated protein like 5, and intersectin 1 as candidate markers in pooled exudates of critically colonised wounds. However, it is unclear whether mRNAs or proteins of the candidate genes would be suitable as biomarkers in fresh exudate. Therefore, this study aimed to evaluate the validity of the mRNAs and proteins as fresh exudate markers for critical colonisation. Three wound models of normal healing, critical colonisation, and infection were created in rats. Fresh swab‐collected exudates were collected, and mRNA and protein expression levels were measured. In the fresh wound exudates, the detection frequency of Itgb6 tended to decrease in the critically colonised and infected wounds (P = .067), and those of Cpne4 and Nppb tended to be lower in the infected wounds than in the normal healing and critically colonised wounds (P = .006 and .067, respectively). In contrast, there was no difference in protein expression in the exudates. This study suggests that Itgb6 mRNA in fresh exudates is a promising biomarker for critical colonisation.
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影响因子：
3.7
作者：
Huang L;Minematsu T;Kitamura A;Quinetti PC;Nakagami G;Mugita Y;Oe M;Noguchi H;Mori T;Sanada H
通讯作者：
Sanada H
影响因子：
6.9
作者：
Braga, I. A.;Pirett, C. C. N. S.;Diogo Filho, A.
通讯作者：
Diogo Filho, A.
影响因子：
2.9
作者：
Matsui, Yuko;Furue, Masutaka;Miyachi, Yoshiki
通讯作者：
Miyachi, Yoshiki
DOI：
10.1097/00005373-197602000-00002
发表时间：
1976-01-01
影响因子：
--
作者：
LEVINE, NS;LINDBERG, RB;PRUITT, BA
通讯作者：
PRUITT, BA
DOI：
10.1111/j.1532-5415.2009.02307.x
发表时间：
2009-07-01
影响因子：
6.3
作者：
Gorecki, Claudia;Brown, Julia M.;Nixon, Jane
通讯作者：
Nixon, Jane