Resistively heated gas chromatography coupled to quadrupole mass spectrometry
Resistively heated gas chromatography coupled to quadrupole mass spectrometry
复制标题
电阻加热气相色谱法与四极杆质谱联用
DOI：
--
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
U. Brinkman
中科院分区：
文献类型：
--
作者：
J. Dallüge;R. Vreuls;D. Iperen;M. V. Rijn;U. Brinkman
将程序升温气相色谱与四极杆质谱联用，研究了快速程序升温气相色谱的潜力。使用EZ Flash GC，其产生高达1200 ° C/min的温度斜坡并在约30秒内从300 °C冷却至50°C;样品通过常规分流/不分流进样器以两种进样模式进样。一个短的窄孔柱Imm ID）、高气体流速和快速温度程序通常将30分钟的常规分析时间减少到约3分钟。（在m/z 50-310范围内16个光谱/s）被发现足以适当重建色谱峰，并且可以获得高质量的质谱。在一个峰上进行六次扫描足以进行峰积分。该系统的线性范围为0.01-3 ng的注射质量和检测限农药的范围为1-25 pg的全扫描和0.1-5 pg的选择离子监测模式。分析了苹果和鼹鼠肝脏提取物系列，以证明EZ Flash GC的高样品通量和快速筛选的适用性。
The potential of fast temperature-programmed gas chromatography (GC) was studied by combining a resistively heated gas chromatograph and a quadrupole mass spectrometer (MS). An EZ Flash GC was used, which generates temperature ramps up to 1200 /min and cools down from 300 to 50°C in some 30 s; samples were injected via a conventional split/splitless injector in both injection modes. The combination of a short narrow-bore column (5 m x 0.1 mm ID), a high gas flow rate, and fast temperature programs typically decreased conventional analysis times of 30 min to about 3 min. The scan speed of the quadrupole mass spectrometer (16 spectra/s in the range m/z 50-310) was found to be sufficient for a proper reconstruction of the chromatographic peaks, and good-quality mass spectra could be obtained. Six scans across a peak were sufficient for peak integration. The system was linear in the range of 0.01-3 ng injected mass and detection limits for pesticides were in the range of 1-25 pg in the full-scan and 0.1-5 pg in the selected ion monitoring mode. Series of apple and mole liver extracts were analyzed to demonstrate the high sample throughput and suitability of EZ Flash GC for fast screening.