Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing
Self-driven filter-based blood plasma separator microfluidic chip for point-of-care testing
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DOI：
10.1088/1758-5090/7/2/025007
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发表时间：
2015-06-01
期刊：
影响因子：
9
通讯作者：
Mohammadi, Mahdi
中科院分区：
文献类型：
--
作者：
Madadi, Hojjat;Casals-Terre, Jasmina;Mohammadi, Mahdi
目前，对用于临床分析和诊断的芯片实验室设备的需求日益增长，特别是在患者护理领域。大多数血液分析的第一步是从全血中提取血浆。本文介绍了一种新型的、自驱动的血浆分离微流控芯片，它可以从一滴未稀释的新鲜人血中提取超过0.1 μ l的血浆（类似于5 μ l）。该体积的血浆是在合理的时间范围内（3至5分钟）从高纯度（98%以上）的全血中提取的，并且不需要任何外力。这将是实现一次性自我血液检测的第一步，与现有耗时的传统血液分析相比，这种血液检测不需要任何外力或电源来提供和分析新鲜的全血样本。原型在聚二甲基硅氧烷中生产，聚二甲基硅氧烷已用强非离子表面活性剂（Silwet L-77）改性，以实现亲水性。这种微流控芯片设计的主要优点是过滤区域的堵塞延迟，这导致提取的血浆量增加（0.1 μ l）。此外，血浆可以收集在一个或多个10 μ m深的通道中，以便于多种血液分析的检测和读出。这种高容量的血浆提取是由于一种新颖的设计，通过使用不同的流体动力学原理，如收缩效应和对称过滤模式，在不干扰红细胞轨迹的情况下结合了最大的泵送效率。为了证明微流控芯片的功能，我们设计并制造了一种新型的混合微器件，表现出微流控和侧流免疫层析测试的好处。所提出的混合微器件的性能进行了验证，使用快速检测促甲状腺激素在一个单一的滴全血。
There is currently a growing need for lab-on-a-chip devices for use in clinical analysis and diagnostics, especially in the area of patient care. The first step in most blood assays is plasma extraction from whole blood. This paper presents a novel, self-driven blood plasma separation microfluidic chip, which can extract more than 0.1 mu l plasma from a single droplet of undiluted fresh human blood (similar to 5 mu l). This volume of blood plasma is extracted from whole blood with high purity (more than 98%) in a reasonable time frame (3 to 5 min), and without the need for any external force. This would be the first step towards the realization of a single-use, self-blood test that does not require any external force or power source to deliver and analyze a fresh whole-blood sample, in contrast to the existing time-consuming conventional blood analysis. The prototypes are manufactured in polydimethylsiloxane that has been modified with a strong nonionic surfactant (Silwet L-77) to achieve hydrophilic behavior. The main advantage of this microfluidic chip design is the clogging delay in the filtration area, which results in an increased amount of extracted plasma (0.1 mu l). Moreover, the plasma can be collected in one or more 10 mu m-deep channels to facilitate the detection and readout of multiple blood assays. This high volume of extracted plasma is achieved thanks to a novel design that combines maximum pumping efficiency without disturbing the red blood cells' trajectory through the use of different hydrodynamic principles, such as a constriction effect and a symmetrical filtration mode. To demonstrate the microfluidic chip's functionality, we designed and fabricated a novel hybrid microdevice that exhibits the benefits of both microfluidics and lateral flow immunochromatographic tests. The performance of the presented hybrid microdevice is validated using rapid detection of thyroid stimulating hormone within a single droplet of whole blood.