MATURE HUMAN EOSINOPHILS HAVE THE CAPACITY TO EXPRESS HLA-DR
MATURE HUMAN EOSINOPHILS HAVE THE CAPACITY TO EXPRESS HLA-DR
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DOI：
10.1073/pnas.86.4.1348
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发表时间：
1989-02-01
影响因子：
11.1
通讯作者：
WELLER, PF
中科院分区：
文献类型：
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作者：
LUCEY, DR;NICHOLSONWELLER, A;WELLER, PF
已知人嗜酸性粒细胞在成熟时失去Ia抗原表达，因此，从5个嗜酸性粒细胞供体和4个正常供体中的3个的血液中获得的嗜酸性粒细胞不能显示主要组织相容性复合体II类抗原HLA-DR，如通过流式细胞术测定的。然而，当来自这9个供体的嗜酸性粒细胞与重组人粒细胞-巨噬细胞集落刺激因子和鼠3 T3成纤维细胞一起培养时，HLA-DR始终在嗜酸性粒细胞上发展。到培养的第4-6天，24-97%的嗜酸性粒细胞是HLA-DR+，并且嗜酸性粒细胞保持形态成熟。与此相反，另一种II类抗原，HLA-DQ，是不能检测的9个捐助者的8个嗜酸性粒细胞的流式细胞术。培养的嗜酸性粒细胞能够合成HLA-DR，如通过将[35 S]甲硫氨酸掺入免疫可沉淀的HLA-DR重链和轻链所证明的。这些发现表明，成熟的嗜酸性粒细胞可以合成和表达HLA-DR，并提供了一种手段，使嗜酸性粒细胞可以与CD 4+淋巴细胞相互作用。
Human eosinophils are known to lose Ia antigen expression as they mature, and, accordingly, eosinophils obtained from the blood of five eosinophilic donors and three of four normal donors failed to display the major histocompatibility complex class II antigen HLA-DR, as determined by flow cytometry. However, when eosinophils from these nine donors were maintained in culture with recombinant human granulocyte-macrophage colony-stimulating factor and murine 3T3 fibroblasts, HLA-DR consistently developed on the eosinophils. By days 4-6 of culture, 24-97% of eosinophils were HLA-DR+, and the eosinophils remained morphologically mature. In contrast, another class II antigen, HLA-DQ, was not detectable by flow cytometry on eosinophils from eight of nine donors. Cultured eosinophils were able to synthesize HLA-DR, as documented by the incorporation of [35S]methionine into immunoprecipitable HLA-DR heavy and light chains. These findings show that mature eosinophils can synthesize and express HLA-DR and provide a means whereby eosinophils may interact with CD4+ lymphocytes.