The retina of Manduca sexta: rhodopsin expression, the mosaic of green-, blue- and UV-sensitive photoreceptors, and regional specialization
The retina of Manduca sexta: rhodopsin expression, the mosaic of green-, blue- and UV-sensitive photoreceptors, and regional specialization
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DOI：
10.1242/jeb.00571
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发表时间：
2003-10
影响因子：
2.8
通讯作者：
Richard H. White;Huihong Xu;T. Münch;R. R. Bennett-R.;Erin A Grable
中科院分区：
文献类型：
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作者：
Richard H. White;Huihong Xu;T. Münch;R. R. Bennett-R.;Erin A Grable
通过免疫细胞化学验证了Manduca sexta复眼中单个光感受器的光谱灵敏度，并使用针对三种鉴定的视紫红质（P520，P450和P357）的氨基末端序列的多克隆抗血清绘制了视网膜镶嵌图。视网膜由一个小的近端细胞和七个或八个延伸穿过视网膜的细长细胞组成。在每个小网膜中，一个或两个细长的dv细胞取向的背腹轴的视网膜晶格表达P450或P357。前后轴和斜轴上的6个细长的ap和ob细胞表达P520。小近端pr细胞似乎也表达P520。视网膜镶嵌区分为三个不同的领域：腹侧和背侧域，分为主要的视网膜，和一个大的背缘区域。免疫细胞化学的数据提供了一个高分辨率的地图，Manduca视网膜，确认和细化早期的低分辨率ERG光谱灵敏度测量。背侧和腹侧区域，在赤道边界分开，通过蓝色敏感的dv细胞比例的差异来区分：这些细胞在腹侧视网膜占主导地位，但在背侧视网膜中较少。对绿色敏感的ap和ob受体均匀分布在背侧和腹侧区域，而对紫外线敏感的dv细胞分布相当均匀，因为背侧区域的许多小视网膜仅包含一个dv细胞。类似地，背缘小网膜仅包含dv对受体的腹侧成员，其中三分之二表达P357。否则，背缘受体不表达三种已测序的Manduca视蛋白中的任何一种;它们必须表达尚未克隆的视紫红质。
SUMMARY Spectral sensitivities of individual photoreceptors in the compound eye of Manduca sexta were verified by immunocytochemistry, and the retinal mosaic was mapped, using polyclonal antisera raised against amino-terminal sequences of three identified rhodopsins: P520, P450 and P357. Retinulae are composed of a small proximal cell and seven or eight elongate cells extending across the retina. In each retinula, one or two elongate dv cells oriented in the dorsal-ventral axis of the retinal lattice express either P450 or P357. Six elongate ap and ob cells in the anterior-posterior and oblique axes express P520. The small proximal pr cell also appears to express P520. The retinal mosaic is regionalized into three distinct domains: ventral and dorsal domains that divide the main retina, and a large dorsal rim area. The immunocytochemical data provide a high-resolution map of the Manduca retina that confirms and refines earlier low-resolution ERG spectral sensitivity measurements. The dorsal and ventral domains, separated at a well-defined equatorial border, are distinguished by differences in the proportion of blue-sensitive dv cells: these cells dominate the ventral retina but are less abundant in the dorsal retina. Green-sensitive ap and ob receptors are uniformly distributed across the dorsal and ventral domains, and UV-sensitive dv cells are fairly uniformly distributed because many retinulae in the dorsal domain contain only one dv cell. Similarly, dorsal rim retinulae contain only the ventral member of the dv pair of receptors, two-thirds of which express P357. Otherwise, dorsal rim receptors express none of the three sequenced Manduca opsins; they must express rhodopsins that have yet to be cloned.