A SIMPLE THEORETICAL TREATMENT OF A COMPETITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) AND ITS APPLICATION TO THE DETECTION OF HUMAN BLOOD-GROUP ANTIGENS
A SIMPLE THEORETICAL TREATMENT OF A COMPETITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) AND ITS APPLICATION TO THE DETECTION OF HUMAN BLOOD-GROUP ANTIGENS
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DOI：
10.1016/0022-1759(90)90237-p
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发表时间：
1990-07-20
影响因子：
2.2
通讯作者：
JONES, MN
中科院分区：
文献类型：
--
作者：
CHAPMAN, V;FLETCHER, SM;JONES, MN
已经发展了一种理论来解释竞争性酶联免疫吸附试验(ELISA)的行为，其中固定的抗原与液体相的抗原竞争限量的抗体。抗体与抗原在固体表面(微滴定井)上的结合用结合常数k的朗缪尔吸附来描述，提出了两个方程来描述作为竞争抗原浓度的函数的ELISA信号的行为：一个精确方程和一个近似方程，当固定化抗原的表面覆盖率未知时可使用该方程。结果表明，竞争抗原浓度与酶联免疫吸附试验信号的关系曲线取决于抗体的K/k。这一理论已经用几种与卵巢囊液A物质竞争的固定化A血型抗原进行了测试，并发现可以充分描述这些竞争的ELISA，其检测下限约为1 ng血型抗原。
A theory has been developed to explain the behavior of a competitive enzyme-linked immunosorbent assay (ELISA) in which an immobilized antigen competes with a liquid-phase antigen for a limiting amount of antibody. The binding of antibody to antigen on a solid surface (microtitre well) is described in terms of Langmuirian adsorption with a binding constant k. Two equations are presented to describe the behaviour of the ELISA signal as a function of competing antigen concentration; an exact equation and an approximate equation which can be used when the surface coverage of the immobilized antigen is not known. It is shown how curve of ELISA signal vs. competing antigen concentration depend on K/k[antibody]. The theory has been tested using several immobilized blood group A antigens competing with ovarian cyst fluid A substance and found to adequately describe these competitive ELISAs which have a detection limit of approximately 1 ng of blood group antigen.