Estrogenic effects of herbal medicines from Costa Rica used for the management of menopausal symptoms.
Estrogenic effects of herbal medicines from Costa Rica used for the management of menopausal symptoms.
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DOI：
10.1097/gme.0b013e3181a4c76a
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Mahady GB
中科院分区：
文献类型：
--
作者：
Doyle BJ;Frasor J;Bellows LE;Locklear TD;Perez A;Gomez-Laurito J;Mahady GB
Outcomes from the Women's Health Initiative have demonstrated adverse effects associated with hormone therapy (HT), and have prioritized the need to develop new alternative treatments for the management of menopause and osteoporosis. To this end, we have been investigating natural herbal medicines used by Costa Rican women to manage menopausal symptoms. Seventeen plant species were collected and extracted in Costa Rica. To establish possible mechanisms of action, and determine their potential future use for menopause or osteoporosis, the estrogenic activities of the herbal extracts were investigated in an estrogen reporter gene ERβ-CALUX® assay in U2-OS cells, and in reporter and endogenous gene assays in MCF-7 cells. Six of the plant extracts bound to the estrogen receptors. Four of the six extracts stimulated reporter gene expression in the ERβ-CALUX® assay. All six extracts modulated expression of endogenous genes in MCF-7 cells, with four extracts acting as estrogen agonists and two extracts, Pimenta dioica and Smilax domingensis, acting as partial agonist/antagonists by enhancing E2-stimulated pS2 mRNA expression, but reducing E2-stimulated PR and PTGES mRNA expression. Both P. dioica and S. domingensis induced a 2ERE-luciferase reporter gene in transient transfected MCF-7 cells, which was inhibited by the ER antagonist ICI 182780. This work presents a plausible mechanism of action for many of the herbal medicines used by Costa Rican women to treat menopausal symptoms. However, it further suggests that studies of safety and efficacy are needed before these herbs should be used as alternative therapies to HT.
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影响因子：
5.4
作者：
Michel, Joanna L.;Mahady, Gail B.;Caceres, Armando
通讯作者：
Caceres, Armando
影响因子：
3.8
作者：
Sonneveld, E;Jansen, HJ;van der Burg, B
通讯作者：
van der Burg, B
影响因子：
4.8
作者：
Chang, Edmund C.;Frasor, Jonna;Katzenellenbogen, Benita S.
通讯作者：
Katzenellenbogen, Benita S.
影响因子：
5.4
作者：
Michel, Joanna;Duarte, Reinel Eduardo;Mahady, Gail B.
通讯作者：
Mahady, Gail B.
DOI：
10.1016/s0005-7916(97)00030-x
发表时间：
1997-12-01
影响因子：
1.8
作者：
Wijma, K;Melin, A;Hammar, M
通讯作者：
Hammar, M