A simplified design for the C. elegans lifespan machine.
A simplified design for the C. elegans lifespan machine.
复制标题
DOI：
10.14440/jbm.2020.332
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Driscoll M
中科院分区：
文献类型：
--
作者：
Abbott M;Banse SA;Melentijevic I;Jarrett CM;Ange JS;Sedore CA;Falkowski R;Blue BW;Coleman-Hulbert AL;Johnson E;Guo M;Lithgow GJ;Phillips PC;Driscoll M
秀丽线虫寿命测定是研究长寿基本生物学的一种广泛使用的方法。传统的线虫寿命分析需要对单个动物进行劳动密集型的微观监测，以评估几周内的生死情况，这使得大规模的高通量研究变得不切实际。由Stroustrup等人开发的寿命机器。(2013)采用了平板扫描仪技术，以促进线虫存活分析效率的重大技术进步。引入了一个平台，在该平台中，寿命分析的很大一部分是自动化的，使得寿命研究的范围与以前的完全手动分析和促进的新发现是不可能的。然而，正如最初所描述的那样，建造和操作基于扫描仪的寿命机器需要相当大的努力和专业知识。在这里，我们报告了简化结构、降低成本、消除某些机械故障并减少分析工作量要求的设计改进。我们记录的修改应该会让感兴趣的实验室更容易接触到寿命机器。
Caenorhabditis elegans (C. elegans) lifespan assays constitute a broadly used approach for investigating the fundamental biology of longevity. Traditional C. elegans lifespan assays require labor-intensive microscopic monitoring of individual animals to evaluate life/death over a period of weeks, making large-scale high throughput studies impractical. The lifespan machine developed by Stroustrup et al. (2013) adapted flatbed scanner technologies to contribute a major technical advance in the efficiency of C. elegans survival assays. Introducing a platform in which large portions of a lifespan assay are automated enabled longevity studies of a scope not possible with previous exclusively manual assays and facilitated novel discovery. Still, as initially described, constructing and operating scanner-based lifespan machines requires considerable effort and expertise. Here we report on design modifications that simplify construction, decrease cost, eliminate certain mechanical failures, and decrease assay workload requirements. The modifications we document should make the lifespan machine more accessible to interested laboratories.