ACTIVATION OF KI-RAS 2 GENE IN HUMAN-COLON AND LUNG CARCINOMAS BY 2 DIFFERENT POINT MUTATIONS
ACTIVATION OF KI-RAS 2 GENE IN HUMAN-COLON AND LUNG CARCINOMAS BY 2 DIFFERENT POINT MUTATIONS
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DOI：
10.1038/304507a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
GOEDDEL, DV
中科院分区：
文献类型：
--
作者：
CAPON, DJ;SEEBURG, PH;GOEDDEL, DV
Kirsten (Ki)-ras c[互补]DNA 克隆是从表达激活的 c-Ki-ras2 基因的人肺癌和结肠癌细胞系中制备的。 DNA序列分析和转染研究表明，同一密码子的不同点突变可以激活该基因；大多数人类 c-Ki-ras2 mRNA 使用与病毒对应物不同的第四个编码外显子的序列；并且至少 1 个细胞系对于激活的基因在功能上是纯合的。
Kirsten (Ki)-ras c[complementary]DNA clones were prepared from human lung and colon carcinoma cell lines expressing an activated c-Ki-ras2 gene. DNA sequence analysis and transfection studies indicate that different point mutations at the same codon can activate the gene; that most human c-Ki-ras2 mRNA uses sequences from a 4th coding exon distinct from that of its viral counterpart; and that at least 1 cell line is functionally homozygous for the activated gene.