CENTRIOLE CYCLE IN SYNCHRONIZED HELA CELLS
CENTRIOLE CYCLE IN SYNCHRONIZED HELA CELLS
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DOI：
10.1083/jcb.36.2.329
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发表时间：
1968-01-01
影响因子：
7.8
通讯作者：
MICALI, A
中科院分区：
文献类型：
--
作者：
ROBBINS, E;JENTZSCH, G;MICALI, A
HeLa细胞在其生命周期中的进展伴随着中心粒复制和中心粒周围的变化，这些变化与DNA合成和有丝分裂同步。准备复制的第一个迹象发生在G1期间，此时两个正交的中心粒分离。出芽复制开始于/或接近DNA合成起始，由G2完成。心泡周围的变化可能与梭形小管的形成有因果关系，包括囊泡、电子不透明体和无定形的心泡周围晕。这些现象在前期后期开始消失，有丝分裂的其余部分表现为中心粒和中心周活性下降。
Progression of the HeLa cell through its life cycle is accompanied by centriolar replication and pericentriolar changes that are in synchrony with DNA synthesis and mitosis. The 1st signs of preparation for replication occur during G1 at which time the 2 orthogonal centrioles separate. Replication by budding begins at/or near the initiation of DNA synthesis and is completed by G2. Pericentriolar changes which probably are causally related to spindle tubule formation occur at this time and include the appearance of vesicles, electron-opaque bodies, and an amorphous pericentriolar halo. These phenomena begin to disappear by late prophase, and the remainder of mitosis manifests decreasing centriolar and pericentriolar activity.