Variable osteogenic performance of MC3T3-E1 subclones impacts their utility as models of osteoblast biology
Variable osteogenic performance of MC3T3-E1 subclones impacts their utility as models of osteoblast biology
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DOI：
10.1038/s41598-019-44575-8
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发表时间：
2019-06-05
影响因子：
4.6
通讯作者：
Horton, Jason A.
中科院分区：
文献类型：
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作者：
Hwang, Phillip W.;Horton, Jason A.
自发永生化的小鼠颅骨细胞系MC 3 T3-E1及其衍生的亚克隆是成骨细胞生物学的广泛使用的模型。许多研究人员在看似相似的实验条件下报告了相互矛盾的数据，尽管研究的具体亚克隆通常没有具体说明。本研究旨在直接比较市售MC 3 T3-E1亚克隆4、14和24对成骨诱导培养基和/或rhPTH刺激的反应性[1-34]。我们检测了成骨基因表达、胶原基质存款和矿化的能力以及PTH 1 R的表达和信号传导功能。我们的数据表明，每个亚克隆承担很少的功能相似的其他人，或主要颅骨成骨细胞。具体地说，亚克隆4对PTH刺激有反应并能够进行基质矿化，而亚克隆14和24不能忠实地复制成骨细胞生物学的这些关键方面。此外，亚克隆4和原代颅骨成骨细胞的基因表达谱之间几乎没有重叠。我们使用这些细胞系的经验表明，MC 3 T3-E1衍生的细胞系是成骨细胞生物学的不完美模型，并加强了明确阐述研究材料的选择和报告的重要性。
The spontaneously immortalized murine calvarial cell line MC3T3-E1 and its derivative subclones are widely used models of osteoblast biology. Many investigators have reported conflicting data under seemingly similar experimental conditions, though the specific subclone studied is often not specified. The purpose of this study was to directly compare the commercially available MC3T3-E1 subclones 4, 14, and 24 in terms of responsiveness to osteogenic induction media and/or stimulation with rhPTH[1-34]. We assayed osteogenic gene expression, capacity to deposit and mineralize a collagenous matrix, and the expression and signaling function of PTH1R. Our data demonstrate that each subclone bears little functional resemblance to the others, or to primary calvarial osteoblasts. Specifically, whereas subclone 4 is responsive to PTH stimulation and capable of matrix mineralization, subclones 14 and 24 do not faithfully replicate these key aspects of osteoblast biology. Furthermore, little overlap was observed between the gene expression profile of subclone 4 and primary calvarial osteoblasts. Our experience working with these cell lines demonstrates that the MC3T3-E1 derived cell lines are imperfect models of osteoblast biology, and reinforce the importance of clearly articulating selection and reporting of research materials.