Beta-tricalcium phosphate granules improve osteogenesis in vitro and establish innovative osteo-regenerators for bone tissue engineering in vivo.
Beta-tricalcium phosphate granules improve osteogenesis in vitro and establish innovative osteo-regenerators for bone tissue engineering in vivo.
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β-磷酸三钙颗粒可改善体外成骨作用，并为体内骨组织工程建立创新的骨再生剂
DOI：
10.1038/srep23367
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发表时间：
2016-03-22
影响因子：
4.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Gao P;Zhang H;Liu Y;Fan B;Li X;Xiao X;Lan P;Li M;Geng L;Liu D;Yuan Y;Lian Q;Lu J;Guo Z;Wang Z
传统的骨缺损治疗方法的弊端促使骨组织工程的探索。本研究旨在探索适合骨再生的β-磷酸三钙(β-TcP)颗粒，为构建β-TcP基骨再生器提供一种有效的方法。对直径分别为1 mm和1~2.5 mm的TCP颗粒进行体外评价。将具有良好成骨性能的β-TcP颗粒用于建立体内生物反应器，称为骨再生器，采用两种不同的方法制备。与1 mm颗粒相比，1~2.5 mMβ-tCP可促进骨髓间充质干细胞的增殖、葡萄糖消耗和碱性磷酸酶活性(P <； 0.0 5)。此外，与1 mMβ-Tcp孵育的BMSCs相比，1-2.5 mMβ-tCP孵育的骨髓间充质干细胞的矮小相关转录因子2、碱性磷酸酶、骨钙素、骨桥蛋白和1型胶原蛋白以及成骨相关蛋白碱性磷酸酶、1型胶原和矮小相关转录因子2的表达水平显著高于1 mMβ-tCP孵育的骨髓间充质干细胞(P <； 0.05)。荧光标记、显微计算机断层扫描和组织学染色分析表明，与骨膜切开的骨再生器相比，穿透股骨的两孔骨再生器促进骨再生的作用显著增强(P <； 005)。这项研究提供了一种生物功能化生物反应器的替代方案，显示出更好的成骨能力。
The drawbacks of traditional bone-defect treatments have prompted the exploration of bone tissue engineering. This study aimed to explore suitable β-tricalcium phosphate (β-TCP) granules for bone regeneration and identify an efficient method to establish β-TCP-based osteo-regenerators. β-TCP granules with diameters of 1 mm and 1–2.5 mm were evaluated in vitro. The β-TCP granules with superior osteogenic properties were used to establish in vivo bioreactors, referred to as osteo-regenerators, which were fabricated using two different methods. Improved proliferation of bone mesenchymal stem cells (BMSCs), glucose consumption and ALP activity were observed for 1–2.5 mm β-TCP compared with 1-mm granules (P < 0.05). In addition, BMSCs incubated with 1–2.5 mm β-TCP expressed significantly higher levels of the genes for runt-related transcription factor-2, alkaline phosphatase, osteocalcin, osteopontin, and collagen type-1 and the osteogenesis-related proteins alkaline phosphatase, collagen type-1 and runt-related transcription factor-2 compared with BMSCs incubated with 1 mm β-TCP (P < 0.05). Fluorochrome labelling, micro-computed tomography and histological staining analyses indicated that the osteo-regenerator with two holes perforating the femur promoted significantly greater bone regeneration compared with the osteo-regenerator with a periosteum incision (P < 0.05). This study provides an alternative to biofunctionalized bioreactors that exhibits improved osteogenesis.