Comparison of Anodic Community in Microbial Fuel Cells with Iron Oxide-Reducing Community
Comparison of Anodic Community in Microbial Fuel Cells with Iron Oxide-Reducing Community
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DOI：
10.4014/jmb.1510.10037
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发表时间：
2016-04-01
影响因子：
2.8
通讯作者：
Yamashita, Takahiro
中科院分区：
文献类型：
--
作者：
Yokoyama, Hiroshi;Ishida, Mitsuyoshi;Yamashita, Takahiro
Fe（III）氧化物还原细菌包括产电细菌，它们具有将电子转移到细胞外不溶性电子受体的类似性质。产电细菌可以利用微生物燃料电池（MFC）的阳极作为末端电子受体进行乙酸盐的厌氧氧化。在本研究中，阳极社区进行了比较，社区使用Fe（III）氧化物（水铁矿）作为电子受体加上乙酸盐氧化。为了精确地分析结构，使用用于MFC的相同接种物通过富集培养物建立群落。高通量测序的16S rRNA基因显示阳极社区的结构和Fe（III）的氧化还原社区之间存在相当大的差异。在阳极群落中主要检测到地被虫物种（>46%）。与此相反，假单胞菌（70%）和Desulfosporosinus（16%）占主导地位的Fe（III）氧化还原社区。这些结果表明，Geodynamic物种是最专门的Fe（III）还原细菌的电子转移到MFC的阳极。此外，本研究表明，存在一个新的谱系的细菌属假单胞菌，高度喜欢水铁矿作为终端电子受体在乙酸氧化。
The group of Fe(III) oxide-reducing bacteria includes exoelectrogenic bacteria, and they possess similar properties of transferring electrons to extracellular insoluble-electron acceptors. The exoelectrogenic bacteria can use the anode in microbial fuel cells (MFCs) as the terminal electron acceptor in anaerobic acetate oxidation. In the present study, the anodic community was compared with the community using Fe(III) oxide (ferrihydrite) as the electron acceptor coupled with acetate oxidation. To precisely analyze the structures, the community was established by enrichment cultures using the same inoculum used for the MFCs. High-throughput sequencing of the 16S rRNA gene revealed considerable differences between the structure of the anodic communities and that of the Fe(III) oxide-reducing community. Geobacter species were predominantly detected (>46%) in the anodic communities. In contrast, Pseudomonas (70%) and Desulfosporosinus (16%) were predominant in the Fe(III) oxide-reducing community. These results demonstrated that Geobacter species are the most specialized among Fe(III)-reducing bacteria for electron transfer to the anode in MFCs. In addition, the present study indicates the presence of a novel lineage of bacteria in the genus Pseudomonas that highly prefers ferrihydrite as the terminal electron acceptor in acetate oxidation.