Selective interactions of concanavalin A‐modified tetraether lipid liposomes
Selective interactions of concanavalin A‐modified tetraether lipid liposomes
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刀豆球蛋白 A 修饰的四醚脂质体的选择性相互作用
DOI：
10.1002/pssc.201001175
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Bakowsky U.
中科院分区：
文献类型：
--
作者：
Ozcetin A;Dayyoub E;Hobler Ch;Keusgen M;Bakowsky U.
位点特异性脂质体的高稳定性和长循环特性对于改善药物的靶向性和给药系统以及提高高效生物活性药物的生物利用度具有重要意义。四醚脂是从嗜酸热浆菌中提取的一种非常稳定的脂类。由于四醚脂质体具有良好的化学稳定性和生物相容性，是一种理想的脂质体给药载体。本研究以伴刀豆球蛋白A（ConA）为模型蛋白，模拟了甘露聚糖表面的特异性吸附。刀豆球蛋白A（ConA）是从刀豆中提取的一种凝集素，它与炎症活性组织或肿瘤上的糖结构域相互作用。在这项研究中，稳定的TEL脂质体的制备和表征。此外，为了开发侧特异性脂质体，活化四醚脂质，并且将ConA共价键合到所制备的脂质体的表面上。通过反射干涉光谱（RifS）的生物分子相互作用分析（BIA）研究了ConA缀合脂质体与甘露聚糖修饰表面的相互作用。这提供了碳水化合物结构和TEL脂质体之间相互作用的真实的观察。在RifS测量之后，还通过AFM成像可视化了特异性相互作用。结果表明，ConA偶联脂质体具有较高的特异性吸附效率。因此，ConA和甘露聚糖表面之间的这种特异性吸附测定可以被认为是利用特异性生物标志物进行进一步研究的模型，用于选择性活性剂转移到肿瘤和炎症活性组织。（© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA，魏因海姆）
For the development of site specific liposomes, high stability and long circulation properties are very promising for the improvement of drug targeting and drug delivery system as well as improvement of bioavailability of efficient bioactive drugs. Tetraether lipids (TELs) are very stabile lipids, extracted fromThermoplasma acidophilum. Because of its high chemical stability and its biocompatibility, Tetraether lipid liposomes prepared with TELs are excellent candidates for liposomal drug delivery. In this study a model protein, Concanavalin A (ConA) was chosen for a simulation of a specific adsorbtion onto mannan surface. Concanavalin A (ConA) is a lectin, extracted fromCanavalia ensiformis, which interacts with sugar domains localized on inflammatory active tissues or tumors. In this study, stable TEL liposomes were prepared and characterized. Furthermore to develop side specific liposomes, tetraether lipids were activated and, ConA was covalently bond onto the surface of the prepared liposomes. Interaction of ConA conjugated liposomes with mannan‐modified surfaces was investigated by Biomolecular Interaction Analysis (BIA) via Reflectometric Interference Spectroscopy (RIfS). This provides real time observation of interactions between carbohydrate structure and TEL liposomes. Specific interaction was also visualised by AFM imaging subsequent to the RIfS measurements. According to the results, with ConA conjugated liposomes, high specific adsorption efficiency was observed. Consequently, this specific adsorption assay between ConA and mannan surface can be considered as a model for the further studies utilized with specific biomarkers for a selective active agent transfer to tumors and inflammatory active tissues. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)