Picolinic acid spray stimulates the antioxidative metabolism and minimizes impairments on photosynthesis on wheat leaves infected by Pyricularia oryzae
Picolinic acid spray stimulates the antioxidative metabolism and minimizes impairments on photosynthesis on wheat leaves infected by Pyricularia oryzae
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DOI：
10.1111/ppl.12917
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发表时间：
2019-12-01
影响因子：
6.4
通讯作者：
Rodrigues, Fabricio Avila
中科院分区：
文献类型：
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作者：
Aucique-Perez, Carlos Eduardo;Resende, Renata Sousa;Rodrigues, Fabricio Avila
真菌病原体产生毒素，这些毒素对于它们的致病性和/或对它们的宿主的侵袭性是重要的。吡啶甲酸（PA）是一种非寄主选择性毒素，能引起水稻叶片类似于稻瘟病菌侵染的病变。鉴于非寄主选择性毒素在植物病害防治中的应用价值，本研究探讨了叶面喷施PA对小麦（Triticum aestivum L.）在非植物毒性浓度下，植物可以提高其对稻瘟病的抵抗力，刺激抗氧化代谢，并尽量减少光合作用的变化。浓度大于0.1 mgml（-1）的PA喷雾导致叶片病变，损害光合作用，并与过氧化氢（H2 O2）和超氧阴离子自由基（O-2（中心点-））的更大积累有关。0.3mg/ml的PA抑制菌丝生长、分生孢子产生和萌发。与未喷洒的植株相比，在真菌接种前24小时喷洒PA（0.1 mgml（-1））的植株在接种后72和96小时分别显著降低了稻瘟病严重程度59%和23%。稻瘟病症状的减少与抗坏血酸过氧化物酶（EC 1.11.1.11）、过氧化氢酶（EC 1.11.1.6）、谷胱甘肽过氧化物酶（EC 1.11.1.9）、谷胱甘肽还原酶（EC www.example.com）、谷胱甘肽-S-转移酶（EC 2.5.1.18）、过氧化物酶（EC1.15.1.1）和超氧化物歧化酶（EC 1.11.1.7）活性的增加、H2 O2和O-2（中心点-）积累的减少、丙二醛产生的减少以及光合器官受损的减少有关。1.8.1.7一个更有效的抗氧化代谢，迅速清除活性氧在P. lasticum感染，而不显着降低光合性能，是一个显着的效果，获得PA喷雾。
Fungal pathogens produce toxins that are important for their pathogenesis and/or aggressiveness towards their hosts. Picolinic acid (PA), a non-host selective toxin, causes lesions on rice leaves resembling those originated from Pyricularia oryzae infection. Considering that non-host selective toxins can be useful for plant diseases control, this study investigated whether the foliar spray with PA on wheat (Triticum aestivum L.) plants, in a non-phytotoxic concentration, could increase their resistance to blast, stimulate the anti-oxidative metabolism, and minimize alterations in photosynthesis. The PA spray at concentrations greater than 0.1 mgml(-1) caused foliar lesions, compromised the photosynthesis and was linked with greater accumulation of hydrogen peroxide (H2O2) and superoxide anion radical (O-2(center dot-)). Fungal mycelial growth, conidia production and germination decreased by PA at 0.3 mgml(-1). Blast severity was significantly reduced by 59 and 23%, respectively, at 72 and 96h after inoculation for plants sprayed with PA (0.1 mgml(-1)) at 24h before fungal inoculation compared to non-sprayed plants. Reduction on blast symptoms was linked with increases on ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6), glutathione peroxidase (EC 1.11.1.9), glutathione reductase (EC 1.8.1.7), glutathione-S-transferase (EC 2.5.1.18), peroxidase (EC 1.11.1.7), and superoxide dismutase (EC 1.15.1.1) activities, lower H2O2 and O-2(center dot-) accumulation, reduced malondialdehyde production as well as less impairments to the photosynthetic apparatus. A more efficient antioxidative metabolism that rapidly scavenges the reactive oxygen species generated during P. oryzae infection, without dramatically decreasing the photosynthetic performance, was a remarkable effect obtained with PA spray.