Identification of Domestic Rice Cultivars by RAPD Method Using Milled Rice Grains as Samples.
Identification of Domestic Rice Cultivars by RAPD Method Using Milled Rice Grains as Samples.
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以精米粒为样品的 RAPD 法鉴定国产水稻品种。
DOI：
10.3136/nskkk.44.386
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发表时间：
1997
期刊：
影响因子：
0.2
通讯作者：
S. Kawasaki
中科院分区：
文献类型：
--
作者：
K. Ohtsubo;T. Fujii;Yoichi Hashino;H. Toyoshima;H. Okadome;Sumiko Nakamura;T. Fuse;S. Kawasaki
由于稻米包装袋上必须标明品种、产地和生产年份，因此，用客观方法鉴别品种、产地和生产年份的技术必不可少。对几个农业试验站生产的稻米进行RAPD分析。每个DNA通过CTAB法提取并通过PCR方法使用10 mer引物扩增。通过对日本10个水稻主栽品种的PCR产物进行分析，可以对水稻主栽品种进行鉴定。
As it is obliged that cultivars, location and year of production should be displayed on bag of milled rice grains, technology to identify them by the objective methods is indispensable. Milled rice grains produced at the several agricultural experiment stations were subjected to the RAPD method. Each DNA was extracted by CTAB method and amplified by PCR method using 10 mer primers. It became possible to identify the rice cultivars using milled rice grains as samples by the electrophore-sis of the PCR products among the dominant 10 rice cultivars in Japan.