Resolving the spatial kinetics of electric pulse-induced ion release
Resolving the spatial kinetics of electric pulse-induced ion release
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DOI：
10.1016/j.bbrc.2012.06.055
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发表时间：
2012-07-13
影响因子：
3.1
通讯作者：
Ibey, Bennett L.
中科院分区：
文献类型：
--
作者：
Beier, Hope T.;Roth, Caleb C.;Ibey, Bennett L.
细胞暴露于纳秒脉冲电场（nsPEF）会导致细胞内钙的迅速增加。这种钙离子爆发的机制尚不清楚，但假设是通过纳米孔直接涌入，激活特定的离子通道，或直接破坏细胞器。然而，很可能涉及/激活了几种机制，从而导致难以通过慢速成像方法分离的复杂事件链。在这封信中，我们描述了一种新的高速成像系统，能够确定nsPEF暴露后单个细胞内钙爆发的空间位置。啮齿动物神经母细胞瘤细胞的初步数据显示，该系统能够在暴露几毫秒内跟踪体外钙爆发的位置。这些数据表明，钙离子从最靠近电极（极）的质膜区域进入细胞，并且在细胞外钙缺乏的情况下发生细胞内钙释放。我们相信这种新技术将使我们能够在时间和空间上分离各种nspef诱导的效应，从而对电场与细胞膜之间相互作用的机制产生强有力的见解。(C) 2012爱思唯尔公司版权所有。
Exposure of cells to nanosecond pulsed electric fields (nsPEF) causes a rapid increase in intracellular calcium. The mechanism(s) responsible for this calcium burst remains unknown, but is hypothesized to be from direct influx through nanopores, the activation of specific ion channels, or direct disruption of organelles. It is likely, however, that several mechanisms are involved/activated, thereby resulting in a complex chain of events that are difficult to separate by slow imaging methods. In this letter, we describe a novel high-speed imaging system capable of determining the spatial location of calcium bursts within a single cell following nsPEF exposure. Preliminary data in rodent neuroblastoma cells are presented, demonstrating the ability of this system to track the location of calcium bursts in vitro within milliseconds of exposure. These data reveal that calcium ions enter the cell from the plasma membrane regions closest to the electrodes (poles), and that intracellular calcium release occurs in the absence of extracellular calcium. We believe that this novel technique will allow us to temporally and spatially separate various nsPEF-induced effects, leading to powerful insights into the mechanism(s) of interaction between electric fields and cellular membranes. (C) 2012 Elsevier Inc. All rights reserved.