Role of fission yeast Tup1-like repressors and Prr1 transcription factor in response to salt stress
Role of fission yeast Tup1-like repressors and Prr1 transcription factor in response to salt stress
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DOI：
10.1091/mbc.01-12-0568
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发表时间：
2002-09-01
影响因子：
3.3
通讯作者：
Whitehall, SK
中科院分区：
文献类型：
--
作者：
Greenall, A;Hadcroft, AP;Whitehall, SK
在粟酒裂殖酵母中，Sty1 丝裂原激活蛋白激酶和 Atf1 转录因子控制转录诱导，以响应盐浓度升高。在此，我们证明两种阻遏蛋白 Tup11 和 Tup12 以及 Prr1 转录因子也在盐休克反应中发挥作用。我们发现，同时删除两个 tup 基因会导致对升高的阳离子浓度（K+ 和 Ca2+）过敏，并且我们将编码细胞内阳离子转运蛋白的 cta3(+) 鉴定为一种新型应激基因，其表达受 Sty1 途径正向控制，并受 Tup 阻遏物负向调节。 cta3(+) 的表达通过 Tup 阻遏蛋白维持在低水平，而缓解抑制需要 Sty1、Atf1 和 Prr1。 Prr1 也是 KCl 介导的几个其他 Sty1 依赖性基因（例如 gpxl(+) 和 ctt1(+)）的诱导所必需的。令人惊讶的是，在 tup11Delta tup12Delta 突变体中，KCI 介导的 cta3(+) 表达诱导独立于 Styl 发生，因此 Tup 抑制子将诱导与 Styl 途径联系起来。我们还报道，与许多其他 Sty1 和 Atf1 依赖性基因相比，cta3(+) 的表达仅由高盐浓度诱导。然而，在缺乏 Tup 阻遏物的情况下，这种特异性就会丧失，并且一系列应激会诱导 cta3(+) 表达。
In Schizosaccharomyces pombe, the Sty1 mitogen-activated protein kinase and the Atf1 transcription factor control transcriptional induction in response to elevated salt concentrations. Herein, we demonstrate that two repressors, Tup11 and Tup12, and the Prr1 transcription factor also function in the response to salt shock. We find that deletion of both tup genes together results in hypersensitivity to elevated cation concentrations (K+ and Ca2+) and we identify cta3(+), which encodes an intracellular cation transporter, as a novel stress gene whose expression is positively controlled by the Sty1 pathway and negatively regulated by Tup repressors. The expression of cta3(+) is maintained at low levels by the Tup repressors, and relief from repression requires the Sty1, Atf1, and Prr1. Prr1 is also required for KCl-mediated induction of several other Sty1-dependent genes such as gpxl(+) and ctt1(+). Surprisingly, the KCI-mediated induction of cta3(+) expression occurs independently of Styl in a tup11Delta tup12Delta mutant and so the Tup repressors link induction to the Styl pathway. We also report that in contrast to a number of other Sty1- and Atf1-dependent genes, the expression of cta3(+) is induced only by high salt concentrations. However, in the absence of the Tup repressors this specificity is lost and a range of stresses induces cta3(+) expression.