Revisiting the role of sucrose in PLGA-PEG nanocarrier for potential intranasal delivery
Revisiting the role of sucrose in PLGA-PEG nanocarrier for potential intranasal delivery
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DOI：
10.1080/10837450.2017.1287731
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发表时间：
2018-01-01
影响因子：
3.4
通讯作者：
Puglisi, G.
中科院分区：
文献类型：
--
作者：
Bonaccorso, A.;Musumeci, T.;Puglisi, G.
纳米载体的有效设计是一个重大挑战，必须与给药途径相关。研究鼻内途径用于局部、全身或脑治疗。为了开发具有鼻内递送的合适性质的纳米载体，以实现脑和销售产品，在原材料方面简化制剂是极其重要的。通常添加表面活性剂和冷冻保护剂以改善聚合物纳米颗粒的结构化和/或储存。通过纳米沉淀法制备PLGA-PEG纳米载体，评估蔗糖作为表面活性剂样和冷冻保护剂的关键作用，目的是获得比其他论文中提出的更简单的制剂。光子相关光谱和Turbiscan分析表明，蔗糖是一个有用的赋形剂在制备过程中，它有效地冷冻保护纳米粒子。在所研究的具有不同程度的PEG的纳米载体中，PEG化的PLGA（5%）赋予纳米颗粒与粘蛋白之间的弱相互作用，如通过热分析和粘蛋白颗粒方法所证明的。此外，对作为上皮细胞系的HT 29的体外生物学研究在所有检测浓度下均未显示该纳米载体的细胞毒性作用。选择的纳米系统也进行了研究，以负载多西他赛，作为模型药物，并通过技术的角度来看，其特征在于。
The efficient design of nanocarriers is a major challenge and must be correlated with the route of administration. Intranasal route is studied for local, systemic or cerebral treatments. In order to develop nanocarriers with suitable properties for intranasal delivery, to achieve brain and to market the product, it is extremely important the simplification of the formulation in terms of raw materials. Surfactants and cryoprotectants are often added to improve structuration and/or storage of polymeric nanoparticles. PLGA-PEG nanocarriers were prepared by nanoprecipitation method evaluating the critical role of sucrose as surfactant-like and cryoprotectant, with the aim to obtain a simpler formulation compared to those proposed in other papers. Photon correlation spectroscopy and Turbiscan analysis show that sucrose is a useful excipient during the preparation process and it effectively cryoprotects nanoparticles. Among the investigated nanocarriers with different degree of PEG, PEGylated PLGA (5%) confers weak interaction between nanoparticles and mucin as demonstrated by thermal analysis and mucin particle method. Furthermore, in vitro biological studies on HT29, as epithelium cell line, does not show cytotoxicity effect for this nanocarrier at all texted concentrations. The selected nanosystem was also studied to load docetaxel, as model drug, and characterized by a technological point of view.