Label-free DNA-based biosensors using structure-selective light-up dyes
Label-free DNA-based biosensors using structure-selective light-up dyes
复制标题
使用结构选择性发光染料的无标记 DNA 生物传感器
作者：
Guo Yahui;Xu Lijun;Hong Shanni;Sun Qingqing;Yao Weirong;Pei Renjun
无标记生物传感器（LFB）在经济高效的应用中显示出巨大的潜力，大多数基于DNA的LFB是基于结合诱导结构转变的原理。本文综述了近年来利用结构选择性核酸染料制备DNA基LFB的研究进展。在这篇评论中的集合已经结构化的基础上选择性结合的染料对特定的DNA构象，包括单链DNA，双链DNA，三链体DNA，i-基序和G-四链体。最新的研究采用多功能核酸酶，迷人的纳米材料，逻辑门和级联，DNA连接和纳米结构也已收集作为例子。预测了LFB的发展趋势，即对LFB的灵敏度、分析智能化、检测可靠性、便携快速等方面的迫切需求将推动LFB的持续发展。
Label-free biosensors (LFBs) have demonstrated a great potential in cost-effective applications, and most of the DNA-based LFBs are based on the principle of binding-induced structural transformation. This review is a collection of the latest reported studies, which have employed structure-selective nucleic acid dyes for the development of DNA-based LFBs. The collections in this review have been structured based on the selective binding of dyes towards specific DNA conformations, including single-stranded DNA, double-stranded DNA, triplex DNA, i-motifs and G-quadruplexes. The newest studies of employing versatile nucleases, fascinating nanomaterials, logic gates and cascades, DNA junctions and nanostructures have also been collected as examples. It is predicted that the imperative requirement for ultrahigh sensitivity, intelligent analysis, reliable detection, portable and fast assay would force the LFB development in a stimulative and continuous way.