A stochastic model for DNA translocation through an electropore.
A stochastic model for DNA translocation through an electropore.
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DOI：
10.1016/j.bbamem.2012.05.025
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Miao Yu;Wenchang Tan;Hao Lin
中科院分区：
文献类型：
--
作者：
Miao Yu;Wenchang Tan;Hao Lin
A 1D Fokker–Planck simulation of DNA translocation through an electropore under finite pulses is presented. This study is motivated by applications relevant to DNA electrotransfer into biological cells via electroporation. The results review important insights. The translocation may occur on two disparate time scales, the electrophoretic time (~ms), and the diffusive time (~s), depending on the pulse length. Furthermore, a power-law correlation is observed, F-PST~(Vmtp)a/Nb, where F-PST is the final probability of successful translocation, Vmis the transmembrane potential, tpis the pulse length, and N is the DNA length in segments. The values for a and b are close to 1 and 1.5, respectively. The simulated results are compared with previous data to interpret the trends. In particular, the diffusive time scale is used to explain the frequency dependence observed in electroporation experiments with uni- and bi-polar pulse trains. The predictions from the current model can be harnessed to help design experiments for the further understanding and quantification of DNA electrotransfer.
