Analyses of Pigment Compositions and Anthocyanin Biosynthesis Gene Expression in Hirado Azalea Cultivars
Analyses of Pigment Compositions and Anthocyanin Biosynthesis Gene Expression in Hirado Azalea Cultivars
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DOI：
10.2503/hortj.utd-142
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发表时间：
2020-01-01
影响因子：
1.2
通讯作者：
Nakatsuka, Akira
中科院分区：
文献类型：
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作者：
Meanchainiboon, Sunisa;Kobayashi, Nobuo;Nakatsuka, Akira
平户杜鹃（Hirado azalea）是一种大型开花植物，粗糙杜鹃（Rhododendron scabrum）、R.里彭斯河x mucronatum和其他相关品种被认为是它的父母。本研究通过分析平户杜鹃花色素组成模式和花色素苷生物合成基因的表达，研究了平户杜鹃品种与野生种和老品种的相关性。平户杜鹃品种根据色素组成分为4类。只含矢车菊素衍生物的平户杜鹃品种，其花朵颜色与红杜鹃相似。粗糙。同时含有矢车菊素和飞燕草素衍生物以及黄酮醇的平户杜鹃品种产生的花色与R。ripense和R.大萼只含黄酮醇的平户杜鹃品种，花朵呈白色，与R.白柳含有花青素衍生物和黄酮醇的平户杜鹃品种与其亲本相比，花色范围更广。所有样品均表达F3 ′ H、DFR和ANS基因，如通过实时定量RT-PCR所确定的。然而，F3 '5'H基因仅在含有飞燕草素衍生物的样品中表达。此外，“Shiroryukyu”也表达了所有四个基因，就像彩色花的品种一样，即使它的花是白色。这些结果表明，平户杜鹃与R. scabrum为基础，可能会产生广泛的花色除了红色，由于存在的F3 '5'H基因，从R。里彭斯河macrosepalum和'Shiro-ryukyu'。
The Hirado azalea is a large flowering plant and Rhododendron scabrum, R. ripense, R. x mucronatum, and other related cultivars are considered to be its parents. In this study, we investigated the correlation of Hirado azalea cultivars with wild species and old cultivars by analyzing anthocyanidin composition patterns and the expression of anthocyanin biosynthesis genes. Hirado azalea cultivars were divided into four groups according to their pigment compositions. Hirado azalea cultivars with only cyanidin derivatives had red colored flowers similar to those of R. scabrum. Hirado azalea cultivars with both cyanidin and delphinidin derivatives as well as flavonol produced similar flower colors to those of R. ripense and R. macrosepalum. Hirado azalea cultivars with only flavonol had white colored flowers similar to those of R. mucronatum 'Shiro-ryukyu'. Hirado azalea cultivars with cyanidin derivatives and flavonol exhibited a wider range of flower colors compared to their parents. All samples expressed F3'H, DFR, and ANS genes, as determined by real-time quantitative RT-PCR. However, the F3'5'H gene was expressed only in samples containing delphinidin derivatives. Moreover, 'Shiroryukyu' also expressed all four genes, as did cultivars with colored flowers, even though its flowers are white. These results suggested that the hybridization of Hirado azalea using R. scabrum as the base may produce a wide range of flower colors besides red owing to the presence of the F3'5'H gene from R. ripense, R. macrosepalum, and 'Shiro-ryukyu'.