Ca2+ release by inositol trisphosphate from Ca2+-transporting microsomes derived from uterine sarcoplasmic reticulum.
Ca2+ release by inositol trisphosphate from Ca2+-transporting microsomes derived from uterine sarcoplasmic reticulum.
复制标题
肌醇三磷酸从来自子宫肌浆网的 Ca2 转运微粒体释放 Ca2。
DOI：
10.1016/0006-291x(85)91718-8
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发表时间：
1985
影响因子：
3.1
通讯作者：
J. Miller
中科院分区：
文献类型：
--
作者：
M. E. Carsten;J. Miller
来自妊娠子宫肌浆网的微粒体，通过差速离心和蔗糖密度梯度离心分离，在ATP存在下积累Ca 2+。三磷酸肌醇引起释放的钙离子，在剂量依赖性的方式。离子载体A23187释放的Ca ~（2+）中有40%被5 μ M三磷酸肌醇释放。在添加三磷酸肌醇之前用EDTA去除镁并没有改变Ca 2+的释放过程。这些结果表明，通过动员细胞内Ca ~（2+），三磷酸肌醇可能是激素刺激和平滑肌收缩之间的联系。
Microsomes derived from pregnant uterine sarcoplasmic reticulum, isolated by differential and sucrose density gradient centrifugation, accumulates Ca2+in the presence of ATP. Inositol trisphosphate caused release of this Ca2+, in a dose dependent manner. 40% of the Ca2+that can be released by the ionophore A23187 was released by 5 uM inositol trisphosphate. Removal of Mg by EDTA prior to addition of inositol trisphosphate did not change the course of Ca2+release. These results indicate that by mobilizing intracellular Ca2+, inositol trisphosphate may be the link between hormonal stimuli and smooth muscle contraction.