Self-aspirating atmospheric pressure chemical ionization source for direct sampling of analytes on surfaces and in liquid solutions
Self-aspirating atmospheric pressure chemical ionization source for direct sampling of analytes on surfaces and in liquid solutions
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DOI：
10.1002/rcm.2062
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发表时间：
2005-01-01
影响因子：
2
通讯作者：
Van Berkel, GJ
中科院分区：
文献类型：
--
作者：
Asano, KG;Ford, MJ;Van Berkel, GJ
介绍了一种自吸式加热雾化器探头，可用于大气压化学电离(APCIMS)直接分析表面和液体样品中的分析物。使用利血平和黄体酮作为测试化合物，展示了该探针作为自吸APCI源的功能和性能。通过扫描反相薄层色谱板的展开线，首先证明了该探针直接从表面采样分析物的有效性，在该板中发现了三组分的染料混合物，即脂肪红7B、溶剂绿3和溶剂蓝35，并分离了这些组分。通过计算机控制的采样探头(x，y平面)扫描显影通道，同时使用四极离子陷阱质谱仪记录全扫描质谱图。此外，还利用串联质谱仪(MS/MS)对药物片剂(特强泰诺(R)和伊维斯塔(R)片)进行了表面采样，并对其有效成分(对乙酰氨基酚和雷洛昔芬)进行了选择性检测。最后，以添加了可替宁-d(3)的可替宁标准品为内标，说明了从384孔微滴定板的孔中采样分析物溶液并使用MS/MS检测进行定量分析的能力。2005年由约翰·威利父子有限公司出版。
A self-aspirating heated nebulizer probe is described and demonstrated for use in the direct analysis of analytes on surfaces and in liquid samples by atmospheric pressure chemical ionization (APCI) mass spectrometry. Functionality and performance of the probe as a self-aspirating APCI source is demonstrated using reserpine and progesterone as test compounds. The utility of the probe to sample analytes directly from surfaces was demonstrated first by scanning development lanes of a reversed-phase thin-layer chromatography plate in which a three-component dye mixture, viz., Fat Red 7B, Solvent Green 3, and Solvent Blue 35, was spotted and the components were separated. Development lanes were scanned by the sampling probe operated under computer control (x, y plane) while full-scan mass spectra were recorded using a quadrupole ion trap mass spectrometer. In addition, the ability to sample the surface of pharmaceutical tablets (viz., Extra Strength Tylenol(R) and Evista(R) tablets) and to detect the active ingredients (acetaminophen and raloxifene, respectively) selectively was demonstrated using tandem mass spectrometry (MS/MS). Finally, the capability to sample analyte solutions from the wells of a 384-well microtiter plate and to perform quantitative analyses using MS/MS detection was illustrated with cotinine standards spiked with cotinine-d(3) as an internal standard. Published in 2005 by John Wiley & Sons, Ltd.