Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus
Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus
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DOI：
10.1016/j.peptides.2010.08.010
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发表时间：
2010-11-01
期刊：
影响因子：
3
通讯作者：
Ueta, Yoichi
中科院分区：
文献类型：
--
作者：
Maruyama, Takashi;Ohbuchi, Toyoaki;Ueta, Yoichi
我们最近开发了一种新的转基因大鼠系，表达精氨酸加压素（AVP）增强的绿色荧光蛋白（eGFP）融合基因。AVP-eGFP转基因在下丘脑的室旁核（PVN）和视上核（SON）以及视交叉上核（SCN）中表达。PVN和SON中的转基因表达对盐负荷和伤害性刺激表现出夸张的反应，然而， SCN 中的 AVP-eGFP 转基因在这些应激条件下没有发生变化 这里我们检查了 SCN 中 AVP-eGFP 转基因与内源性 AVP 和周期（Per1 和 Per2）基因相比的每日表达概况 虽然所有这些基因都在 SCN 中引发了昼夜表达模式，但转基因表达的节律变化率显着大于内源性 AVP 基因 我们还研究了光刺激对 SCN 中 AVP-eGFP 转基因表达的影响AVP-eGFP 转基因大鼠 SCN 中的 AVP Per1 和 Per2 基因 光刺激 90 分钟后 SCN 中 AVP-eGFP mRNA 和 AVP hnRNA 水平显着降低，而 Per2 mRNA 水平显着升高 此外，我们观察了 SCN 中的 eGFP 荧光并记录了游离 SCN eGFP 阳性神经元的电生理特性 AVP-eGFP 转基因大鼠是研究 AVP 昼夜变化和动态的有用动物模型系统并能够在体内和体外轻松识别 SCN AVP 神经元 (C) 2010 Elsevier Inc 保留所有权利
We have recently developed a new transgenic rat line expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene The AVP-eGFP transgene is expressed in the paraventricular (PVN) and supraoptic (SON) nuclei and the suprachiasmatic nucleus (SCN) of the hypothalamus Transgene expression in the PVN and SON showed an exaggerated response to salt loading and nociceptive stimulation However the expression of the AVP-eGFP transgene in the SCN did not change under these stressful conditions Here we examined daily profiles of the expression of the AVP-eGFP transgene in the SCN in comparison with the endogenous AVP and Period (Per1 and Per2) genes While all of these genes elicited diurnal patterns of expression in the SCN the rate of rhythmic change of transgene expression was significantly greater than that of the endogenous AVP gene We also examined the effect of a light stimulus on the expression of the AVP-eGFP AVP Per1 and Per2 genes in the SCN of transgenic rats Ninety minutes after a light stimulus AVP-eGFP mRNA and AVP hnRNA levels in the SCN were significantly decreased while Per2 mRNA levels were significantly increased In addition we observed the eGFP fluorescence in the SCN and recorded the electrophysiological properties of a dissociated SCN eGFP-positive neuron The AVP-eGFP transgenic rat is a useful animal model to study the diurnal change and dynamics of the AVP system and enables the facile identification of SCN AVP neurons both in vivo and in vitro (C) 2010 Elsevier Inc All rights reserved