Acquiring local field potential information from amperometric neurochemical recordings
Acquiring local field potential information from amperometric neurochemical recordings
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DOI：
10.1016/j.jneumeth.2009.01.023
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发表时间：
2009-05-15
影响因子：
3
通讯作者：
Nicolelis, Miguel A. L.
中科院分区：
文献类型：
--
作者：
Zhang, Hao;Lin, Shih-Chieh;Nicolelis, Miguel A. L.
同时获取体内电生理和神经化学信息对于了解内源性神经化学物质如何调节大脑活动的动态至关重要。然而，到目前为止，由于同时实时操作两个独立记录系统的主要技术挑战，这样的任务很少完成。在这里，我们提出了一种更简单的解决方案，仅使用标准电化学技术 - 电流分析法即可实现此目标。为了证明其可行性，我们将电流信号与同时记录的局部场电位 (LFP) 信号进行了比较。我们发现电流信号的高频成分（HFC）并不反映神经化学波动，而是在几个方面类似于LFP，包括：（1）相干光谱波动； (2) 清晰表征不同的大脑状态； (3) 相同的海马θ深度剖面。因此，我们的研究结果首次证明 LFP 和局部神经化学信息可以仅从电化学传感器同时获取。 (C) 2009 Elsevier B.V. 保留所有权利。
Simultaneous acquisition of in vivo electrophysiological and neurochemical information is essential for understanding how endogenous neurochemicals modulate the dynamics of brain activity. However, up to now such a task has rarely been accomplished due to the major technical challenge of operating two independent recording systems simultaneously in real-time. Here we propose a simpler solution for achieving this goal by using only a standard electrochemical technique - amperometry. To demonstrate its feasibility, we compared amperometric signals with simultaneously recorded local field potential (LFP) signals. We found that the high frequency component (HFC) of the amperometric signals did not reflect neurochemical fluctuations, but instead it resembled LFPs in several aspects, including: (1) coherent spectral fluctuations; (2) clear characterization of different brain states; (3) identical hippocampal theta depth profile. As such, our findings provide the first demonstration that both LFP and local neurochemical information can be simultaneously acquired from electrochemical sensors alone. (C) 2009 Elsevier B.V. All rights reserved.