Novel technique for monitoring micturition and sexual function in male rats using telemetry.
Novel technique for monitoring micturition and sexual function in male rats using telemetry.
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使用遥测技术监测雄性大鼠排尿和性功能的新技术。
DOI：
10.1152/ajpregu.00532.2006
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Schmidt,MarkusH
中科院分区：
文献类型：
--
作者：
Nout,YvetteS;Bresnahan,JacquelineC;Culp,Esther;Tovar,CAmy;Beattie,MichaelS;Schmidt,MarkusH
我们开发了一种新技术，通过阴茎海绵体 (CSP) 内的压力监测来同时监测大鼠的排尿和勃起。我们提供了验证该技术的数据，并报告了 10 只清醒、自由活动的雄性大鼠在四种不同行为背景下排尿和勃起事件的压力波形特征。遥测压力传感器被植入 CSP 球内。监测 CSP 压力，同时记录动物的视频以确定排尿的存在和排尿量（n = 7），同时对动物进行行为测试以确定勃起。观察到的排尿和排尿的 CSP 压力波形特征同时发生（r = 0.98），排尿频率为每 24 小时 32 ± 4 次，排尿量为 0.95 ± 0.12 毫升/排尿。由 CSP 压力记录的排尿持续时间与由尿液重量确定的排尿量高度相关 (r= 0.82)。我们发现，在前系结反射勃起测试期间，100% 视觉确认的勃起事件与勃起的 CSP 压力波形特征同时发生。在非接触式勃起和交配测试中，通过遥测技术识别出的勃起比仅通过观察发现的更多。交配测试期间的勃起外观与其他情况下的勃起外观不同；它们的持续时间较短（P < 0.05），并且通常以单个上收缩 CSP 压力峰值为特征，突出了勃起的背景特异性。我们跟踪了 8 周的四只大鼠中，有三只的记录质量保持稳定。我们证明，CSP 压力的遥测记录可以对自由行为的大鼠的阴茎勃起和排尿进行定量和定性评估。
We developed a novel technique to simultaneously monitor micturitions and erections in rats by using pressure monitoring within the corpus spongiosum of the penis (CSP). We present data validating this technique and report pressure waveform characteristics of micturition and erectile events during four different behavioral contexts in 10 awake, freely-moving male rats. Telemetric pressure transducers were implanted in the bulb of the CSP. CSP pressure was monitored while the animals were simultaneously recorded on video for determination of presence and volume (n= 7) of micturitions and while the animals underwent behavioral tests for determination of erections. Observed micturitions and CSP pressure waveforms characteristic of micturitions occurred simultaneously (r= 0.98) at a frequency of 32 ± 4 micturitions per 24 h and with a volume of 0.95 ± 0.12 ml/urination. Micturition duration recorded by CSP pressure and volume determined by urine weight were highly correlated (r= 0.82). We found that 100% of visually confirmed erectile events occurred simultaneously with CSP pressure waveforms characteristic of erections during ex copula reflex erection tests. During noncontact erection and mating tests more erections were identified by telemetry than by observation alone. Erections during mating tests had a different appearance than those seen in other contexts; they were shorter in duration (P< 0.05) and typically were characterized by a single suprasystolic CSP pressure peak, highlighting the context-specificity of erections. Quality of recordings remained stable in three of four rats we followed for 8 wk. We demonstrate that telemetric recording of CSP pressure provides a quantitative and qualitative assessment of penile erections and micturition in freely behaving rats.