A SEMIAUTOMATIC INSTRUMENT FOR RADIOAUTOGRAPHIC COATING TECHNIQUE
A SEMIAUTOMATIC INSTRUMENT FOR RADIOAUTOGRAPHIC COATING TECHNIQUE
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DOI：
10.1177/14.12.923
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
KOPRIWA, BM
中科院分区：
文献类型：
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作者：
KOPRIWA, BM
本文介绍了一种用机械涂敷仪涂敷液态核径迹乳剂的快速、简便的方法，用于定量光镜和电镜放射自显影。在这两种情况下，切片都固定在载玻片上。在垂直固定的一侧浸入熔化的乳液中后，仪器以缓慢、恒定的速度将其取出。结果，样品被涂上一层由均匀分布的银晶体组成的薄而均匀的乳剂层。乳液涂层的厚度可以通过选择乳液稀释度、温度和取出速度的最佳组合来标准化。
By means of a mechanical coating instrument a fast, simple method to coat specimens with liquid nuclear track emulsion has been devised for quantitative light and electron microscopic radioautography. In both cases, the section is mounted on a glass slide. After the vertically held side has been immersed in the melted emulsion, the instrument withdraws it at a slow, constant speed. As a result, the specimen is coated with a thin, uniform emulsion layer composed of homogeneously distributed silver bromide crystals. The thickness of the emulsion coat may be standardized by selection of an optimal combination of emulsion dilution, temperature and withdrawal speed.