Engineered Narrow Size Distribution High Molecular Weight Proteinoids, Proteinoid-Poly(L-Lactic Acid) Copolymers and Nano/Micro-HollowParticles for Biomedical Applications
Engineered Narrow Size Distribution High Molecular Weight Proteinoids, Proteinoid-Poly(L-Lactic Acid) Copolymers and Nano/Micro-HollowParticles for Biomedical Applications
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用于生物医学应用的工程窄尺寸分布高分子量类蛋白、类蛋白-聚（L-乳酸）共聚物和纳米/微米中空颗粒
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
S. Margel
中科院分区：
文献类型：
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作者：
Michal Kolitz;S. Margel
类蛋白质是由氨基酸热缩合形成的不寻常的聚合物。合成了几种类型的类蛋白质，其由一种至三种不同的氨基酸组成，在不存在或存在的情况下，低分子量聚（L-乳酸）（PLLA）。这些蛋白质的聚合动力学，分子量和物理和机械性能进行了阐述。通过使用不同的氨基酸作为结构单元，沿着在其结构中引入PLLA，获得几种高MW耐用类蛋白质的能力产生了生物可降解聚合物和聚合物颗粒的新前景。在合适的温和条件下，类蛋白可以自组装形成相对窄尺寸分布的纳米和微米尺寸的中空颗粒。这种自组装过程用于将不同分子包封在所产生的类蛋白颗粒内。所使用的包封材料之一是吲哚菁绿色（ICG），其是一种已知的且FDA批准的用于医学癌症诊断的近红外染料。测试ICG包封的类蛋白颗粒在小鼠中的生物分布。类蛋白颗粒是无毒和稳定的;因此，它们可能是各种生物医学应用的优异候选物，例如，细胞标记和分离、控制释放、药物靶向等。
Proteinoids are unusual polymers formed by thermal condensation of amino acids. Several types of proteinoids made of one to three different amino acids, in absence or presence, of low molecular weight poly(L-lactic acid) (PLLA), were synthesized. The polymerization kinetics, molecular weights and physical and mechanical properties of these proteinoids were elucidated. The ability to obtain several high-MW durable proteinoids, by using different amino acids as building blocks, along with incorporating PLLA in their structure, yields a new perspective of biodegradable polymers and polymer particles. Under suitable gentle conditions, the proteinoids can self-assemble to form nanoand micron-sized hollow particles of relatively narrow size distribution. This self-assembly process was used for encapsulation of different molecules within the produced proteinoid particles. One of the encapsulated materials used was indocyanine green (ICG), a known and FDA-approved near-IR dye used for medical cancer diagnosis. The ICG-encapsulated proteinoid particles were tested for biodistribution in mice. The proteinoid particles are nontoxic and stable; hence, they may be excellent candidates for various biomedical applications, e.g., cell labeling and separation, controlled release, drug targeting, etc.