Lymphokine activation of T4+ T lymphocytes and monocytes.
Lymphokine activation of T4+ T lymphocytes and monocytes.
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DOI：
10.4049/jimmunol.138.11.3817
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发表时间：
1987-06
影响因子：
4.4
通讯作者：
W. Cruikshank;J. Berman;A. Theodore;J. Bernardo;D. Center
中科院分区：
文献类型：
--
作者：
W. Cruikshank;J. Berman;A. Theodore;J. Bernardo;D. Center
T4抗原是分化的T细胞亚群的标志，其功能尚不清楚。我们先前观察到一种人类趋化因子，淋巴细胞趋化因子(LCF)，可以选择性地诱导未激活的T4+淋巴细胞的运动性反应，这促使我们研究LCF是否也可以诱导T4+细胞的激活。由于LCF选择性地作用于T4+细胞，我们接下来确定T4抗原是否在LCF诱导的细胞激活中起作用。二价抗T4抗体可诱导T4+淋巴细胞的迁移反应，但同一抗体的相应Fab片段不能诱导T4+淋巴细胞的迁移反应。抗T4抗体的Fab片段可阻断LCF和二价抗T4抗体的迁移效应，而抗T3抗体的Fab片段不能阻断LCFs的迁移作用。此外，LCF在24小时内可诱导T4+淋巴细胞表达IL-2受体和HLA-DR抗原，而不是二价抗T4抗体。这些影响在数量上类似于抗T3抗体激活所观察到的效果。LCF诱导的IL-2受体表达可被抗T4抗体和抗T4Fab片段共同孵育所阻断，而抗T4Fab片段不能抑制抗T3激活。由于培养的单核细胞表达T4抗原，我们研究了LCF对培养单核细胞迁移和人类白细胞抗原DR表达的影响。在体外，随着T4抗原表达的增加，LCF和抗T4抗体诱导的单核细胞迁移成比例增加。这种增强的迁移可被抗T4Fab片段抑制。单核细胞活化，通过与LCF孵育24小时后增加的HLA-DR表达来衡量，但不包括抗T4抗体，在数量上与干扰素-γ的效果相似。增强的HLA-DR表达可被抗T4Fab片段阻断，但不能被干扰素-γ抗体阻断。这些研究表明，LCF与T4+淋巴细胞和单核细胞相互作用，诱导细胞迁移和激活。
The function of the T4 antigen, a marker for a differentiated T cell subset, is not well understood. Our previous observation that a chemoattractant human lymphokine, lymphocyte chemoattractant factor (LCF), which selectively induces motile responses in unactivated T4+ lymphocytes, led us to investigate whether LCF could also induce T4+ cell activation. Because LCF acts selectively on T4+ cells, we next determined whether the T4 antigen has a function in this LCF-induced cellular activation. A T4+ lymphocyte migratory response is induced by divalent anti-T4 antibody, but not by corresponding Fab fragments of the same antibody. Fab fragments of anti-T4 antibody, but not Fab fragments of anti-T3 antibody, block the migratory effect of both LCF and divalent anti-T4. Furthermore, LCF but not divalent anti-T4, evokes the expression of interleukin 2 (IL 2) receptors and HLA-DR antigen on T4+ lymphocytes in 24 hr. These effects are quantitatively similar to those observed by anti-T3 antibody activation. LCF-induced IL 2 receptor expression is blocked by co-incubation with anti-T4 antibody and anti-T4 Fab fragments, whereas anti-T3 activation is not inhibitable by anti-T4 Fab fragments. Because cultured monocytes express the T4 antigen, we investigated the action of LCF on cultured monocyte migration and HLA-DR expression. Induction of monocyte migration by LCF and anti-T4 antibody increases proportionally as T4 antigen expression increases in vitro. This enhanced migration is inhibitable by anti-T4 Fab fragments. Monocyte activation, as measured by augmented HLA-DR expression 24 hr after incubation with LCF, but not anti-T4 antibody, is quantitatively similar to the effects of interferon-gamma. Augmented HLA-DR expression is blocked by anti-T4 Fab fragments but not by antibody to interferon-gamma. These studies indicate that LCF interacts with T4+ lymphocytes and monocytes to induce migration and cellular activation.