Opioid growth factor is present in human and mouse gastrointestinal tract and inhibits DNA synthesis.
Opioid growth factor is present in human and mouse gastrointestinal tract and inhibits DNA synthesis.
复制标题
阿片类生长因子存在于人类和小鼠胃肠道中并抑制 DNA 合成。
DOI：
10.1152/ajpregu.1997.272.4.r1094
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
McLaughlin,PJ
中科院分区：
文献类型：
--
作者：
Zagon,IS;Wu,Y;McLaughlin,PJ
天然阿片肽在许多正常和肿瘤细胞和组织中充当生长因子。本研究探讨了阿片类药物对小鼠食管内稳态更新过程中昼夜节律依赖性DNA合成的影响。与06：30和16：00时的标记指数（LI）分别为24.0%和5.5%相反，强效阿片类拮抗剂盐酸纳洛酮（NTX; 10 mg/kg）对小鼠阿片类药物-受体相互作用的破坏导致16：00时食管上皮细胞DNA合成升高49%，但在06：30时无影响。接受[Met 5]脑啡肽（1 mg/kg）给药的小鼠在06：30时的LI较对照水平降低23%，但在16：00时未受影响。这种DNA合成的减少被同时给予纳洛酮（10 mg/kg）阻断;纳洛酮单独给药对细胞复制过程没有影响。在组织培养研究中，NTX和OGF显着增加和减少，分别从对照值的LI。阿片样物质生长因子（OGF）及其受体，ζ，检测到所有，但皮质层的小鼠食管上皮细胞和上皮细胞的胃和小肠和大肠。此外，在人食管上皮的基底细胞和基底上细胞中观察到肽和受体。这些结果表明，内源性阿片肽（OGF）及其受体（zeta）驻留在胃肠上皮细胞，并发挥作用，在细胞更新过程中的张力抑制，直接，昼夜节律依赖的方式。
Native opioid peptides serve as growth factors in a number of normal and neoplastic cells and tissues. This study investigated the influence of opioids on circadian rhythm-dependent DNA synthesis in mouse esophagus during homeostatic renewal. In contrast to a labeling index (LI) of 24.0% at 0630 and 5.5% at 1600, disruption of opioid-receptor interaction by the potent opioid antagonist naltrexone hydrochloride (NTX; 10 mg/kg) in mice resulted in an elevation of 49% in DNA synthesis of esophageal epithelial cells at 1600, but had no effect at 0630. Mice subjected to [Met5]enkephalin (1 mg/kg) had an LI that was decreased 23% from control levels at 0630, but was unaffected at 1600. This decrease in DNA synthesis was blocked by concomitant administration of naloxone (10 mg/kg); naloxone alone had no influence on cell replicative processes. In tissue culture studies, NTX and OGF markedly increased and decreased, respectively, the LI from control values. Both opioid growth factor (OGF) and its receptor, zeta, were detected in all but the cornified layer of mouse esophageal epithelium and in the epithelial cells of the stomach and small and large intestines. In addition, both peptide and receptor were observed in the basal and suprabasal cells of human esophageal epithelium. These results indicate that an endogenous opioid peptide (OGF) and its receptor (zeta) reside in gastrointestinal epithelium and play a role in cellular renewal processes in a tonically inhibitory, direct, and circadian rhythm-dependent fashion.