Limitation of Acinetobacter baumannii treatment by plasmid-mediated carbapenemase ARI-2
Limitation of Acinetobacter baumannii treatment by plasmid-mediated carbapenemase ARI-2
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DOI：
10.1016/s0140-6736(05)78210-6
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发表时间：
1998-01-17
期刊：
影响因子：
168.9
通讯作者：
Amyes, SGB
中科院分区：
文献类型：
--
作者：
Brown, S;Bantar, C;Amyes, SGB
在欧洲，不动杆菌属现在占强化治疗单位（ITU）医院感染的10%。它们总是多重耐药，一个主要问题是碳青霉烯类耐药菌株的发生率不断增加，因为这些抗生素通常是这些感染的治疗选择。南美的碳青霉烯耐药性已经达到了显著影响治疗选择的水平（卡洛斯班塔尔，未发表的数据），这似乎将成为一些欧洲国家的一个问题。ARI-1是一种质粒介导的酶，能够水解亚胺培南，是从该属中分离的第一种碳青霉烯酶。2我们报道了一种新的质粒介导的碳青霉烯酶（ARI-2），来自阿根廷的鲍曼不动杆菌多重耐药临床分离株（首次描述于1996年）。3从支气管肺泡灌洗标本中分离到1622株，用API 20 NE鉴定系统进行鉴定。采用琼脂稀释法测定了一系列抗生素的最低抑菌浓度（MIC）。4制备1622菌株的无细胞提取液，用聚丙烯酰胺凝胶等电聚焦电泳（IEF）检测其β-内酰胺酶带。将抑制剂应用于凝胶以检测酶抑制。通过微生物测定证明亚胺培南水解。2对该测定法进行了改进，以提高灵敏度并定量酶的活性。3固化
Acinetobacter species now account for 10% of nosocomial infections in intensive therapy units (ITU) in Europe. They are invariably multiresistant, and one major concern is the increasing incidence of carbapenem-resistant isolates, since these antibiotics are frequently the therapeutic choice for these infections. Carbapenem resistance in South America has already reached levels that significantly influence therapeutic choices (Carlos Bantar, unpublished data), and this looks set to be a problem in some European countries. 1 ARI-1, a plasmid-mediated enzyme capable of hydrolysing imipenem, was the first carbapenemase to be isolated from this genus. 2 We report a novel plasmid-mediated carbapenemase (ARI-2) from a multiresistant clinical isolate of A baumannii from Argentina (first described in 1996). 3The strain (1622) was isolated from a bronchial alveolar lavage specimen and identification was carried out by the API 20NE identification system. Minimum inhibitory concentrations (MIC) of a range of antibiotics were determined by the agar dilution method. 4 A cell-free extract of strain 1622 was prepared and-lactamase bands were detected by isoelectric focusing (IEF) on a polyacrylamide gel. Inhibitors were applied to the gel to detect enzyme inhibition. Imipenem hydrolysis was demonstrated by a microbiological assay. 2 This assay was modified to increase sensitivity and quantify the activity of the enzyme. 3 Curing