Effects of chemical lipid extraction and arithmetic lipid correction on stable isotope ratios of fish tissues.
Effects of chemical lipid extraction and arithmetic lipid correction on stable isotope ratios of fish tissues.
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DOI：
10.1002/rcm.2347
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发表时间：
2006-02
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影响因子：
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通讯作者：
C. Sweeting;Nicholas Polunin;Simon Jennings
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文献类型：
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作者：
C. Sweeting;Nicholas Polunin;Simon Jennings
为了从碳稳定同位素数据准确解释鱼类营养动力学，有必要提取组织脂质。这是因为组织内和组织间的脂质含量在空间和时间上都不同，并且因为脂质相对于蛋白质是13C耗尽的。然而，脂质提取可能会影响delta15 N，因此需要delta13 C和delta15 N分析的昂贵和耗时的分离。这些问题促使发展的算术校正技术delta13 C，但技术和它们的基本假设还没有得到系统的测试。本研究比较了脂肪提取和算术校正技术对欧洲鲈鱼（Dicentrarchus labrax）组织中δ 13 C和δ 15 N的影响。Folch脂肪提取肌肉和肝脏后，Δ 15 N平均增加0.77 ‰，但富集程度随脂肪含量而变化，因此对Δ 15 N的影响难以预测。未处理和脂质提取样品之间的Δ 13 C和C：N的变化反映了去除的脂质量。质量平衡和脂质校正的算术校正技术对提取脂质的组织的C：N和相对于蛋白质delta13C的脂质delta13C的假定耗尽敏感。然而，在大多数情况下，质量平衡方法适用于大量组织数据的数学校正，前提是可以测定小部分样品的脂质浸提组织的C：N。质量平衡算术校正的应用可以导致显着的时间和成本节省营养动力学研究，因为大多数的delta13 C和delta15 N分析不需要单独运行。
For accurate interpretation of fish trophodynamics from carbon stable isotope data it is necessary to extract tissue lipids. This is because lipid content varies within and among tissues in both space and time, and because lipids are 13C-depleted relative to proteins. However, lipid extraction may affect delta15N, thus requiring costly and time-consuming separation of delta13C and delta15N analyses. These problems have prompted the development of arithmetic correction techniques for delta13C, but the techniques and their underlying assumptions have not been systematically tested. This study compared the effects of lipid extraction and arithmetic correction techniques on delta13C and delta15N of European sea bass (Dicentrarchus labrax) tissues. Following Folch lipid extraction from muscle and liver, there was a mean increase in delta15N of 0.77 per thousand, but enrichment varied with lipid content such that effects on delta15N were hard to predict. Changes in delta13C and C:N between untreated and lipid-extracted samples reflected the quantity of lipid removed. The arithmetic correction techniques of mass balance and lipid correction were sensitive to the C:N of the lipid-extracted tissue and to the assumed depletion of lipid delta13C relative to protein delta13C. However, the mass balance approach was appropriate for the mathematical correction of bulk tissue data in most circumstances, provided that the C:N of lipid-extracted tissue could be determined for a small proportion of samples. Application of mass balance arithmetic correction can lead to significant time and cost savings in trophodynamic studies, because the majority of delta13C and delta15N analyses would not need to be run separately.