Alteration of BMP-4 and Runx2 expression patterns in mouse temporomandibular joint after ovariectomy
Alteration of BMP-4 and Runx2 expression patterns in mouse temporomandibular joint after ovariectomy
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DOI：
10.1111/j.1601-0825.2006.01270.x
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发表时间：
2007-03-01
期刊：
影响因子：
3.8
通讯作者：
Jung, H-S
中科院分区：
文献类型：
--
作者：
Min, H-J;Lee, M-J;Jung, H-S
目的：颞下颌疾病 (TMD) 包括许多涉及咀嚼肌组织或颞下颌关节 (TMJ) 及相关结构的临床病症。先前的研究表明，TMJ 关节软骨中存在高亲和力雌激素受体。本研究的目的是评估雌激素缺乏下小鼠颞下颌关节的发育变化。材料和方法：4个月大的卵巢切除小鼠在几周后处死。我们检查了卵巢切除术后骨形态发生蛋白 (BMP)-4、Runx2 和骨唾液酸蛋白 (BSP) 表达模式的显着变化。结果：对照组各阶段BMP-4、Runx2、BSP表达均无明显差异。在卵巢切除组中，卵巢切除后BMP-4和Runx2表达强度增加。而BSP免疫反应性在2周时略有上升，但随后逐渐下降。结论：雌激素通过调节BMP-4、Runx2和BSP等信号分子在TMJ的代谢和维持中发挥重要作用。我们的结果表明雌激素缺乏是 TMD 的一个可能原因。这项研究进一步揭示了雌激素的成骨特性，可能有助于 TMD 的临床治疗和预防。
OBJECTIVE: Temporomandibular disorder (TMD) includes a number of clinical conditions involving the masticatory musculature or the temporomandibular joint (TMJ) and associated structures. Previous studies have shown the presence of high-affinity estrogen receptors in the TMJ articular cartilage. The aim of this study was to evaluate the developmental changes in mouse TMJ under estrogen deficiency.MATERIALS AND METHODS: Four-month-old ovariectomized mice were killed after certain weeks. We examined the significant alterations of the expression patterns of bone morphogenetic protein (BMP)-4, Runx2, and bone sialoprotein (BSP) after ovariectomy. RESULTS: In the control group, BMP-4, Runx2, and BSP expressions showed no definite difference at any stage. In the ovariectomy group, the intensity of BMP-4 and Runx2 expression increased after ovariectomy. BSP immunoreactivity, however, increased slightly at 2 weeks but then decreased gradually.CONCLUSIONS: Estrogen plays important roles in the metabolism and maintenance of TMJ via regulations of signaling molecules such as BMP-4, Runx2, and BSP. Our results suggest that estrogen deficiency is a candidate cause of TMD. This study revealed further osteogenetic properties of estrogen that may be useful in the clinical treatment and prevention of TMD.