MYC2-Activated TRICHOME BIREFRINGENCE-LIKE37 Acetylates Cell Walls and Enhances Herbivore Resistance
MYC2-Activated TRICHOME BIREFRINGENCE-LIKE37 Acetylates Cell Walls and Enhances Herbivore Resistance
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MYC2 激活的 TRICHOME BIREFRINGENCE-LIKE37 乙酰化细胞壁并增强食草动物抵抗力
DOI：
10.1104/pp.20.00683
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Teng S
中科院分区：
文献类型：
--
作者：
Sun A;Yu B;Zhang Q;Peng Y;Yang J;Sun Y;Qin P;Jia T;Smeekens S;Teng S
多糖的O-乙酰化主要通过改变物理化学性质来修饰植物细胞壁，从而改变细胞壁的结构和功能。细胞壁乙酰化酶的表达调控和特定功能仍有待充分了解。在本研究中，我们克隆了一个拟南芥生长受阻突变体sucrose uncoupled 1（sun 1），sun 1编码TRICHOME BIREFRINGEN-LIKE家族成员AtTBL 37，AtTBL 37在快速生长的植物组织中高表达，编码一个位于高尔基体的蛋白，该蛋白调控次生细胞壁增厚和乙酰化。在sun 1中，茉莉酸信号和下游化学防御基因的表达，包括植物生长蛋白1和支链氨基转移酶4，增加，但出乎意料的是，sun 1更容易受到昆虫取食。茉莉酸信号传导中的中心转录因子MYC 2结合并诱导AtTBL 37表达。MYC 2还促进许多其他TBL的表达。此外，MYC活性增强细胞壁乙酰化。AtTBL 37在myc 2 - 2背景中的过表达减少了食草动物的摄食。我们的研究强调了O-乙酰化在控制植物细胞壁特性、植物发育和草食动物防御中的作用。
O-Acetylation of polysaccharides predominantly modifies plant cell walls by changing the physicochemical properties and, consequently, the structure and function of the cell wall. Expression regulation and specific function of cell wall-acetylating enzymes remain to be fully understood. In this report, we cloned a previously identified stunted growth mutant namedsucrose uncoupled1(sun1) in Arabidopsis (Arabidopsis thaliana).SUN1encodes a member of theTRICHOME BIREFRINGEN-LIKEfamily,AtTBL37.AtTBL37is highly expressed in fast-growing plant tissues and encodes a Golgi apparatus-localized protein that regulates secondary cell wall thickening and acetylation. Insun1, jasmonate signaling and expression of downstream chemical defense genes, includingVEGETATIVE STORAGE PROTEIN1andBRANCHED-CHAIN AMINOTRANSFERASE4, are increased but, unexpectedly,sun1is more susceptible to insect feeding. The central transcription factor in jasmonate signaling, MYC2, binds to and inducesAtTBL37expression. MYC2 also promotes the expression of many otherTBLs. Moreover, MYC activity enhances cell wall acetylation. Overexpression ofAtTBL37in themyc2-2background reduces herbivore feeding. Our study highlights the role ofO-acetylation in controlling plant cell wall properties, plant development, and herbivore defense.