Kinetics of Bartonella birtlesii infection in experimentally infected mice and pathogenic effect on reproductive functions
Kinetics of Bartonella birtlesii infection in experimentally infected mice and pathogenic effect on reproductive functions
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DOI：
10.1128/iai.69.9.5313-5317.2001
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发表时间：
2001-09-01
影响因子：
3.1
通讯作者：
Chomel, BB
中科院分区：
文献类型：
--
作者：
Boulouis, HJ;Barrat, F;Chomel, BB
本文描述了感染伯氏巴尔通体的动力学和对实验小鼠生殖功能的致病作用。B.伯氏杆菌感染，由菌血症确定，发生在BALB/c小鼠静脉接种。低剂量接种（1.5 X 10(3) CFU）仅在75%的小鼠中引起菌血症，而所有小鼠接种高剂量（大于或等于1.5 X 10(4)）。小鼠出现菌血症至少5周（范围5 - 8周），峰值为2 × 10(3)至10(5)CFU/ml血液。处女女性的菌血症水平明显高于男性，但菌血症持续时间相似。在妊娠前感染的小鼠（n = 20），通过计数吸收和妊娠第18天的胎儿死亡来评估胎儿损失。感染小鼠的胎儿死亡和吸收率为36.3%，对照组为14.5% （P < 0.0001）。通过称量活胎来评估胎儿的痛苦。感染小鼠的活胎重量显著低于未感染小鼠（P < 0.0002）。经胎盘传播巴尔通体已被证实，因为76%的胎儿吸收试验培养为伯氏杆菌阳性。感染小鼠胎盘的组织病理学分析显示，母体胎盘有血管病变，这可以解释所观察到的生殖障碍。BALB/c小鼠似乎是研究巴尔通体感染的有用模型。这项研究提供了生殖障碍的第一个证据小鼠实验感染巴尔通体菌株源自野生啮齿动物。
The kinetics of infection and the pathogenic effects on the reproductive function of laboratory mice infected with Bartonella birtlesii recovered from an Apodemus species are described. B. birtlesii infection, as determined by bacteremia, occurred in BALB/c mice inoculated intravenously. Inoculation with a low-dose inoculum (1.5 X 10(3) CFU) induced bacteremia in only 75% of the mice compared to all of the mice inoculated with higher doses (greater than or equal to1.5 X 10(4)). Mice became bacteremic for at least 5 weeks (range, 5 to 8 weeks) with a peak ranging from 2 X 10(3) to 10(5) CFU/ml of blood. The bacteremia level was significantly higher in virgin females than in males but the duration of bacteremia was similar. In mice infected before pregnancy (n = 20), fetal loss was evaluated by enumerating resorption and fetal death on day 18 of gestation. The fetal death and resorption percentage of infected mice was 36.3% versus 14.5% for controls (P < 0.0001). Fetal suffering was evaluated by weighing viable fetuses. The weight of viable fetuses was significantly lower for infected mice than for uninfected mice (P < 0.0002). Transplacental transmission of Bartonella was demonstrated since 76% of the fetal resorptions tested was culture positive for B. birtlesii. The histopathological analysis of the placentas of infected mice showed vascular lesions in the maternal placenta, which could explain the reproductive disorders observed. BALB/c mice appeared to be a useful model for studying Bartonella infection. This study provides the first evidence of reproductive disorders in mice experimentally infected with a Bartonella strain originating from a wild rodent.